大鼠延髓孤束核吻侧段内脑啡肽阳性终末与γ-氨基丁酸阳性神经元联系的形态学研究  

A MORPHOLOGICAL STUDY OF CONNECTIONS BETWEEN ENKEPHALIN IMMUNOREACTIVE TERMINALS AND γ-AMINOBUTYRIC ACID IMMUNOREACTIVE NEURONS IN THE ROSTRAL PORTION OF THE NUCLEUS TRACTUS SOLITARIOUS OF THE RAT

作　　者：刘玲[1] 闫蕴力[1] 赵金平[2] 

机构地区：[1]河北医科大学基础所细胞生物室,石家庄050017 [2]第四军医大学航空航天医学系心理学教研室,西安710032

出　　处：《解剖学报》2007年第2期158-162,共5页Acta Anatomica Sinica

摘　　要：目的观察大鼠孤束核吻侧段(rNTS)内脑啡肽阳性(ENK-ir)终末与γ-氨基丁酸(GABA-ir)阳性神经元之间的联系。方法免疫荧光双重标记技术,包埋前免疫组织化学方法染色结合免疫金颗粒标记的电镜双标技术。结果激光扫描共焦显微镜下可见rNTS内有大量的ENK-ir纤维和终末及散在的GABA-ir神经元。ENK-ir终末与GABA-ir神经元之间形成密切接触。在电镜下可见ENK-ir阳性产物主要定位于圆形清亮突触小泡及大颗粒囊泡表面。ENK-ir轴突终末与GABA-ir及阴性神经元胞体及树突之间形成对称性和非对称性的突触联系,以对称性突触为主。结论rNTS内的ENK-ir终末可能通过抑制或增强GABA能神经元活性,或直接抑制味觉感受神经元活性的方式,参与NTS内味觉信息的感受和调节。Objective To observe the connections between enkephalin immunoreactive （ENK-ir） terminals and γ- aminobutyric acid immunoreactive （GABA-ir） neurons in the rostral portion of the nucleus tractus solltarious （rNTS） of the rat. Methods Double-immunofluorescent labeling method and the pre-embedding immunohistochemical staining combined with immuno-gold particles labeling technique for electron microscopic detection were used in the present study. Results Under the laser scanning confocal microscope, dense ENK-ir fibers and terminals and some GABA-ir neurons were observed in the rNTS. Close contacts between ENK-ir terminals and GABA-ir cell bodies were also detected. By using the electron microscopic technique, ENKoir reaction products were found to be mainly localized on the surface of round clear vesicles and dense-cored vesicles in the axon terminals. ENKoir terminals formed symmetric, which was a dominant pattern, and asymmetric synaptic connections with GABA-ir and immunonegative cell bodies and dendrites. Conclusion The ENK-ir terminals might take part in the transmission and regulation of the taste information in the rNTS through inhibiting, exciting the GABAergic neurons or inhibiting directly the activity of neurons within the rNTS.

关 键 词：脑啡肽 Γ-氨基丁酸 孤束核 味觉 免疫荧光双重标记 免疫电镜双标 大鼠 

分 类 号：R322.81[医药卫生—人体解剖和组织胚胎学]