单侧帕金森病大鼠模型多巴胺系统损害和感觉运动功能改变  

Dopamine System Injury and Sensorimotor Function Change in Unilaterally Parkinson Rat Mode

作　　者：王志忠[1] 田嘉禾[1] 张锦明[1] 王瑞民[1] 郭喆[1] 刘晓飞[1] 

机构地区：[1]解放军总医院核医学科,北京100853

出　　处：《科学技术与工程》2007年第8期1586-1590,共5页Science Technology and Engineering

基　　金：国家863计划重大专项课题(2002AA205081);国家自然科学基金(60331010)资助

摘　　要：为建立完全毁损型偏侧帕金森病大鼠模型,通过影像学和组织学方法观察完全毁损型偏侧帕金森病大鼠模型的多巴胺系统的改变。在中脑内侧束(MFB)两点注射6-羟基多巴(6-OHDA)建立完全毁损型偏侧帕金森病大鼠模型,观察模型大鼠在自然状态下和阿朴吗啡诱导中出现的行为学改变,并通过小动物PET和免疫组织化学的方法观察大鼠脑组织内多巴胺能神经变化。结果帕金森病大鼠模型在行为学上表现了在自发状态下的患肢运动感觉能力降低;在阿朴吗啡作用下患侧肢体的运动感觉能力有所提高,但是协调性较差;组织学和影像学上表现为损毁侧纹状体、黑质、及中脑内侧传导束内多巴胺转运蛋白缺失。说明完全毁损型偏侧帕金森病大鼠模型表现为单侧肢体的运动感觉功能障碍,通过神经学显像技术和组织学方法能探测到损毁侧纹状体内多巴胺能神经末梢的缺失。Order to set up the Completely Unilaterally Lesioned Parkinson Rat Model and investigated the characterization of behavioral and image of model rat, two 6-hydoxydopamine injections into right medial forebrain bundle （MFB） to setup completely unilaterally Parkinson rat model are given and observed their abnormal behavioral under both nature and apomorphine used situation. The ^11C-β-CFT animal-PET to image change of dopamine system is used. Compared with the inactive side, the initiation of stepping movements by the left （contra-lateral）paw is greatly reduced, the initial time was prolonged in the nature situation; under the apomorphine inducing, the rats show function improved of lesioned limb＇. The ^11C-β-CFT image showed the depletion of presynaptic maker DAT in the lesion side. It is conclused completely unilaterally that Parkinson rat model showed extensive neuropathology and profound loss of sensorimotor, and the imagine technology can test the change.

关 键 词：帕金森大鼠模型 正电子发射计算机断层显像(PET) 多巴胺转运蛋白 

分 类 号：R742.5[医药卫生—神经病学与精神病学]