流式细胞仪测定肿瘤细胞内柔红霉素的含量  

Detection of Daunorubicin in Tumor cells by Flow Cytometry

作　　者：祁明芳[1] 黄新智[1] 

机构地区：[1]暨南大学医学院血液病学研究室,暨南大学医学院附属医院内科

出　　处：《暨南大学学报（自然科学与医学版）》1996年第2期32-34,共3页Journal of Jinan University(Natural Science & Medicine Edition)

摘　　要：用流式细胞仪技术测定了Ｋ５６２／Ａ０２多药耐药细胞系和Ｋ５６２／Ｓ药物敏感细胞系细胞内柔红霉素（ＤＮＲ）的含量，发现在２μｍｏｌ／Ｌ的ＤＮＲ孵育条件下，Ｋ５６２／Ａ０２细胞内ＤＮＲ平均道峰值为７２，Ｋ５６２／Ｓ细胞内ＤＮＲ平均道峰值为１１２，细胞内ＤＮＲ含量前者明显低于后者（Ｐ＜０．０１）。结果表明，流式细胞仪技术能较好地检测出细胞系对ＤＮＲ细胞内蓄积的差异，提示此技术可鉴别多药耐药肿瘤细胞和敏感细胞，可用于临床多药耐药肿瘤患者的筛选。Abstract The daunorubicin content in K562/A02 multidrug resistant cell line and K562/S drug sensitive cell line was measured by flow cytometry . The results showed that daunorubicin mean peak channel number in K562/A02 and K562/S was 72 and 112 respectively when incubated with 2 umol/L daunorubicin. The quantity of daunorubicin in K562/A02 was much lower than that in K562/S (P<0.01). These results indicated that flow cytometry revealed the difference of the intracellular daunorubicin conant clearly between two cell lines. It also suggested that this technique was capable of differntiating multidrug resistant tumor cells from sensitive counterparts and is probably very useful in sorting patents with multidrug resistant tumor.Keywonts: multidrug resistance, flow cytometry , daunorubicin

关 键 词：柔红霉素 流式细胞仪 肿瘤细胞 白血病 治疗 

分 类 号：R733.7[医药卫生—肿瘤]