一氧化氮合酶在肝纤维化及肝硬化大鼠肝脏组织中的表达  

Expression of nitric oxide synthase in live tissue of rats with hepatic fibrosis and hepatic cirrhosis

作　　者：刘铭[1] 邢桦云[1] 韩德五[1] 李炳蔚[1] 赵元昌[1] 

机构地区：[1]山西医科大学病理生理学教研室,太原030001

出　　处：《山西医科大学学报》2007年第4期305-308,共4页Journal of Shanxi Medical University

摘　　要：目的探讨一氧化氮合酶在肝纤维化及肝硬化大鼠肝脏组织中的表达。方法将18只Wistar大鼠分为三组:正常对照组(NCG)、复合因素致肝纤维化组(HFG)、肝硬化组(HCG)。观察外周血浆内毒素(ET)、丙氨酸氨基转移酶(ALT)和肝组织匀浆NO水平,免疫组化染色分析内皮型一氧化氮合酶(eNOS)、诱导型一氧化氮合酶(iNOS)在各组肝组织中的表达。结果血浆ET和ALT、肝组织匀浆NO在HF组和HC组明显高于NG组。eNOS和iNOS的表达均为HF组和HC组明显高于NC组,HF组的iNOS表达高于eNOS表达而HC组的eNOS表达高于iNOS表达。直线相关分析肝组织匀浆NO和外周血浆ET呈高度正相关。结论eNOS和iNOS可能都参与了肝纤维化及肝硬化时NO的生成,且肝纤维化时以iNOS生成的NO为主,肝硬化时在NO的生成中eNOS和iNOS都起重要作用,可能与内毒素的诱导有关。Objective To explore the expression of nitric oxide synthase in live tissue of rats with hepatic fibrosis and hepatic cirrhosis. Methods Eighteen Wistar rats were divided into three groups：normal control group（NCG） ,hepatic fibrosis group（HFG） and hepatic cirrhosis group treated with complex pathogens （HCG）. The levels of plasma endotoxin（ET）, alanine aminotrans/erase（ALT） and liver homogenate NO were detected. The expression of endothelial nitric oxide synthase（eNOS） and inducible nitric oxide synthase （iNOS） in liver were observed by immunohistochemistry. Results The levels of ET,ALT and liver homogenate NO in HFG and HCG were higher than those in NG. The expression of eNOS and iNOS in HFG and HCG was higher than those in NG. The expression of iNOS was higher than that of eNOS in HFG, but the expression of iNOS was lower than that of eNOS in HCG. There was statistical significance among them. The liver homogenate NO and plasma ET had significantly positive correlation. Conclusion Both eNOS and iNOS may involve in the releasing of NO in hepatic fibrosis and hepatic cirrhosis. FurtHermore, iNOS plays the main role in hepatic fibrosis, but both iNOS and eNOS play an important role in hepatic cirrhosis, which may be concerned with the inducing of endotoxin.

关 键 词：肝硬化 一氧化氮合酶 内毒素类 一氧化氮 

分 类 号：R575.2[医药卫生—消化系统]