广西壮族自治区隆安县乙型肝炎疫苗全程接种后的免疫记忆研究  

Studies on the status of immune memory after completion of hepatitis B vaccination

作　　者：李艳萍[1] 李荣成[1] 方孔雄[2] 李河民[3] 胡忠玉[3] 何鹏[3] 吴小音[3] 张瑞[3] 梁争论[3] 庄辉[4] 

机构地区：[1]广西壮族自治区疾病预防控制中心,南宁530021 [2]隆安县疾病预防控制中心 [3]中国药品生物制品检定所 [4]北京大学医学部微生物学系

出　　处：《中华流行病学杂志》2007年第4期319-321,共3页Chinese Journal of Epidemiology

基　　金：国家“十五”科技攻关课题资助项目（2004BA718802）

摘　　要：目的了解接种全程乙型肝炎（乙肝）疫苗后的免疫记忆情况。方法1987-1989年出生时接种乙肝血源疫苗的1201名新生儿,以及1996-1999年出生时接种乙肝酵母重组疫苗的2484名新生儿,于2005年随访时检测乙肝表面抗原（HBsAg）、表面抗体（抗-HBs）和核心抗体（抗- HBc）,结果959名3项乙肝病毒（HBV）标志物均阴性,其中228名接种乙肝血源疫苗,731名接种乙肝酵母重组疫苗,于加强免疫1针乙肝酵母重组疫苗后15 d时检测其抗-HBs。此外,随机选择11名加强免疫后无应答和22名有应答者,应用酶免疫斑点法（ELISPOT）测定白细胞介素-2（IL-2）。有初次免疫后抗-HBs定量检测资料者190名,比较其初次免疫和加强免疫后抗-HBs水平。结果加强免疫后,79．82%接种乙肝血源疫苗者抗-HBs阳转,几何平均滴度（GMT）为325．69 mIU/ml;95．62%接种乙肝酵母重组疫苗者抗-HBs阳转,GMT为745．18 mIU/ml。加强免疫后所产生的抗-HBs水平与初次免疫后抗体滴度有关。加强免疫后抗-HBs阳转者的IL-2阳性率（40．91%）也高于无应答者（P＜0．01）。结论在乙肝疫苗初次免疫后,抗-HBs转阴者中,大部分仍具有免疫记忆,仅少部分在长期随访中丧失免疫记忆。因此,在高流行地区,对丧失免疫记忆者应进行乙肝疫苗加强免疫。Objective To study the immune memory in vaccinees after the completion of a full schedule hepatitis B immunization. Methods One thousand and two hundred one infants born in 1987 - 1989 were immunized with 3 doses of plasma derived hepatitis B vaccine, while 2484 newborn babies during 1996-1999 were injected with 3 doses of the yeast recombinant hepatitis B vaccine. All of the infants under observation were tested for HiMAg,anti-HiM and anti-HBc,in 2005. Of 959 individuals negative for anti-HiM（ 〈 10 mlU/ml）, HIMAg and anti-HBc,228 were immunized with plasma-derived vaccine and 731 with yeast recombinant vaccine after birth. All of them were detected for anti-HBs 15 days after a booster of 10μg yeast recombinant vaccine. In addition,interleukin-2（IL-2） was detected in 11 non-responders and 22 responders after boostering, using an enzyme-linked immunospot（ELISPOT）. The anti-FIBs levels of 190 individuals （91 with plasma derived vaccine and 99 with yeast recombinant vaccine）who had had quantitative data on their antibody status after the primary hepatitis t3 vaccination, were compared with that after the boostering. Results Among the individuals who received plasma derived vaccine 16-18 years ago, 79.82% of them showed the signs of immune memory after one booster, with a geometric mean titer （GMT）of 325.69 mIU/ml. Of the individuals who received the yeast recombinant vaccine 6-9 years ago, 95.62% showed immune memory after one booster, with its GMT of 745.18 mIU/ml. Anti-HIM levels induced by the booster were associated with that after the primary immunization. The positive rate of IL-2 was 40.91% in subjects with good immune memory. However, IL-2 was not detected in non-responders after the booster（P〈 0.01）. Conclusion Most of the individuals who had received a completed schedule of primary hepatitis B vaccination and seroconverted from anti-HBs positive to negative,showed the signs of having immune memory after the booster. Only a small proportion of the vaccinees had lost their immune

关 键 词：乙型肝炎病毒 免疫记忆 加强免疫 

分 类 号：R686[医药卫生—骨科学]