CD4^+ CD25^+调节性T细胞在上皮性卵巢癌患者外周血中的表达及临床意义  

The changes of CD4^+ CD25+ T cells and expression of Foxp3 mRNA in peripheral blood mononuclear cell from patients with epithelial ovarian tumor

作　　者：田庚[1] 张炜阳[1] 赵艳晖[1] 卢雪红[2] 朱凤全[1] 

机构地区：[1]吉林大学第二医院妇产科,吉林长春130041 [2]吉林大学第二医院肾内科,吉林长春130041

出　　处：《中国妇幼保健》2007年第11期1499-1501,共3页Maternal and Child Health Care of China

摘　　要：目的:研究上皮性卵巢癌患者外周血中CD4+ CD25+调节性T细胞数量及其相关基因Foxp3 mRNA的表达特点,探讨CD4+CD25+调节性T细胞在诱导肿瘤免疫耐受中的作用。方法:采用流式细胞术检测卵巢浆液性囊腺癌和粘液性囊腺癌患者外周血中CD4+ CD25+调节性T细胞数量变化;采用半定量RT-PCR方法检测卵巢浆液性囊腺癌和粘液性囊腺癌患者外周血PBMC中Foxp3 mRNA表达水平。结果:卵巢浆液性囊腺癌和粘液性囊腺癌患者外周血中CD4+CD25+调节性T细胞数量均明显升高(P<0.05);Foxp3 mRNA在卵巢浆液性囊腺癌和粘液性囊腺癌患者外周血PBMC中表达明显增高(P<0.05)。结论:CD4+ CD25+调节性T细胞可能在卵巢癌免疫耐受中发挥重要作用。Objective： To investigate the changes of CD4^＋CD25^＋ regulator T ceils and Foxp3 mRNA in peripheral blood from the patients with epithelial ovarian tumor. Methods： Flow cytometry was used to analyze the contents of CD4^＋CD25^＋ T cells in peripheral blood mononuclear ceils from patients with serous cystadenocarcinoma, mucinous cystadenocarcinoma and patients without cancer as controls. The expression of Foxp3 mRNA in peripheral blood mononuclear cell was detected by RT - PCR. Results： The population of CD4^ ＋ CD25^ ＋ T cells in peripheral blood mononuclear cell from patients with serous cystad , mucinous cystadenecarcinoma was significantly higher in comparison with that of controls. The expression of Foxp3 in peripheral blood mononuclear enhanced in patients with tumor comparison with that of controls. Conclusion. CD4^＋CD25^＋ regulator T cell may play a important role in the immune tolerance.

关 键 词：CD4^+CD25^+ 调节性T细胞 FOXP3 肿瘤免疫 

分 类 号：R737.31[医药卫生—肿瘤]