时间分辨荧光免疫一步法检测微囊藻毒素的研究  

One Step Time-Resolved Fluorescence Immunoassay for Direct Estimation of Microcystins

作　　者：雷腊梅[1] 韩博平[1] 宋立荣[2] 

机构地区：[1]暨南大学水生生物研究中心,广州510632 [2]中国科学院水生生物研究所,武汉430072

出　　处：《环境科学》2007年第4期872-875,共4页Environmental Science

基　　金：广东省自然科学基金项目(5300418);国家高技术研究发展计划(863)项目(2005AA6010100502);暨南大学引进优秀人才科研启动基金项目

摘　　要：利用稀土元素Eu^3＋标记微囊藻毒素偶联物MCLR—BSA，在固相包被二抗的微孔板上建立了微囊藻毒素的直接竞争时间分辨荧光免疫法．条件优化后Eu^3＋标记物的稀释度为1：50，微囊藻毒素单抗的合适浓度为100ng／mL．该法对微囊藻毒素检测的灵敏度为0．02ng／mL，试剂的测量范围是0．05～10ng／mL，回收率达到94％以上．A direct-competitive time-resolved fluorescence immunoassay（TRFIA） for microcystin detection was established, which was based on europium labeled MCLR-BSA conjugant and microtiter plates coated with anti-mouse IgG. The optimal dilution of europium labeled MCLR-BSA conjugant is 1/50 and most appropriate titration of anti-microcystin-LR （MCLR） monoclonal antibodies is 100 ng/mL. The standard curve under the optimal conditions shows that the quantitative range is from approximately 0.05 ng/mL to 10 ng/mL The detection limit of the method is 0.02 ng/mL for MCLR, recoveries for microcvstin （ 〉 94% ） in auantitative range is satisfactorv.

关 键 词：微囊藻毒素 时间分辨荧光免疫分析 检测 

分 类 号：X173[环境科学与工程—环境科学] X830