促肝细胞再生磷酸酶-3蛋白在5株癌细胞中的表达  

The expression of phosphatase of regenerating liver-3 in five carcinoma cell lines

作　　者：何太平[1] 谢富华[1] 侯庆华[1] 莫丽儿[2] 梁念慈[1] 

机构地区：[1]广东医学院生物化学与分子生物学研究所,广东湛江524023 [2]广东天然药物研究与开发重点实验室,广东湛江524023

出　　处：《广东医学院学报》2007年第1期1-3,共3页Journal of Guangdong Medical College

基　　金：广东省中医药局课题(No.1060044);湛江市科技攻关项目(No.2006C03001);广东医学院青年基金(No.XQ0412)

摘　　要：目的研究促肝细胞再生磷酸酶-3(phosphatase of regenerating liver-3,PRL-3)在5株癌细胞(HO-8910PM、CNE-2Z、A549、JAR和BEL-7402)中的表达情况,为选择合适的细胞株克隆PRL-3基因奠定基础。方法用Western blot分析PRL-3在5株癌细胞(HO-8910PM、CNE-2Z、A549、JAR和BEL-7402)中的表达。结果PRL-3蛋白在5株癌细胞中都有表达:在HO-8910PM与CNE-2Z中呈高度表达,在A549和BEL-7402中呈中等程度表达,而在JAR中呈低度表达。结论PRL-3在5株癌细胞中均有表达,可选择高表达的HO-8910PM或CNE-2Z细胞克隆PRL-3基因。Objective To observe the exgression of phosphatase of regenerating liver-3 （PRL-3） in five cancer cell lines HO- 8910PM, CNE-2Z, A549, JAR and BEL-7402, on which the appropriate cell lines will be chosen to clone PRL-3 gene. Methods Western blot analysis was used to detect the expression of PRL-3. Results The expression of PRL-3 was clearly detected in all the five carcinoma cell lines, with the highest expression in HO-8910PM and CNE-2Z, moderate expression in A549 and BEL- 7402, and the lowest expression in JAR. Conclusion The expression of PRL-3 was high in call lines HO-S910PM and CNE-2Z, which might be suitable to server as the cell line for cloning PRL-3 gene.

关 键 词：肿瘤 转移 表达 PRL-3 

分 类 号：R73-37[医药卫生—肿瘤]