25G经结膜无缝合巩膜切口与常规20G巩膜切口愈合的实验研究  

Experimental Study of the Healing Condition of Sclerotomy Sites after 25-gauge Transconjunctival Sutureless Sclerotomy and 20-gauge Vitrectomy

作　　者：万婷[1] 胡洁[1] 罗燕[1] 徐哲[1] 林少芬[1] 马萍萍[1] 唐仕波[1] 

机构地区：[1]中山大学中山眼科中心中山大学眼科学国家重点实验室,广州510060

出　　处：《眼科学报》2007年第1期37-42,共6页Eye Science

基　　金：2005年度"十五"国家科技攻关项目(2004BA720A14);广东省科技计划项目(社会发展计划)(2004B30901005)

摘　　要：目的:观察比较25G经结膜无缝合巩膜切口与常规20G巩膜切口的愈合情况。方法:版纳实验用小型猪4只,采用随机对照的方法,选择一只眼行25G巩膜切开术(25G组),另一眼行常规20G玻璃体手术(20G组)。术后0d、10d、20d、60d分别观察大体和石腊切片苏木素-伊红(HE)染色,比较两组巩膜切口的愈合情况。结果:25G组的巩膜切口明显小于20G组。术后第1天,20G组结膜充血较25G组明显。25G组的巩膜切口术后10d已基本愈合,仅有少量炎症细胞。20G组的巩膜切口在术后20d仍未完全愈合,有大量的炎症细胞浸润。术后60d两组切口均无炎症细胞浸润。术后,25G组中1个切口,20G组中2个切口可见玻璃体嵌顿;25G组中2个切口,20G组中4个切口可见纤维组织内生。结论:与20G手术相比,25G手术巩膜切口具有创伤小、愈合快、炎症反应轻的优点。Purpose ： To compare the healing condition of sclerotomy sites after 25-gauge transcon- junctival sutureless sclerotomy and conventional 20-gauge vitrectomy in minipigs. Methods： Four minipigs qualified for experiment were included in the study. One eye underwent sclerotomy with 25G trocar （25G group） and the other underwent 20G- PPV （20G group）. The healing processes of sclerotomy sites were observed grossly and by Hematoxylin-Eosin （HE） staining under light microscopy at 0, 10, 20 and 60 days after operation. Results： Compared with 20G group, scleral incisions in 25G group were much smaller. One day after operation, conjunctival hyperemia in 20G group was more severe than that in 25G group. Sclerotomy sites in 25G group mostly healed 10 days after operation with a few inflammation cells. Sclerotomy sites in 20G group had not healed 20 days after operation and were infiltrated by a great number of inflammation cells. One case in 25-gauge group and 2 cases in 20-gauge group had vitreous incarceration. Two cases in 25-gauge group and 4 cases in 20-gauge group had fibrous ingrowth. Conclusion： Compared with 20-gauge vitrectomy, the smaller sclerotomy sites in 25- gauge transconjunctival sutureless sclerotomy heal faster and have lighter inflammation reaction. Vitreous incarceration and proliferation ingrowth could be found in both surgeries. Eye Science 2007; 23 ： 37-42.

关 键 词：20G 25G 巩膜切口 小型猪 

分 类 号：R779.6[医药卫生—眼科]