清洁造纸用菌株的形态、酶系分析及其生物漂白  

Biobleaching of Bamboo Kraft Pulp with the Enzymes Produced from a Fungus

作　　者：莫佳琳[1] 付时雨[1] 詹怀宇[1] 闵江马[1] 

机构地区：[1]华南理工大学制浆造纸工程国家重点实验室,广东广州510640

出　　处：《中国造纸学报》2007年第1期23-27,共5页Transactions of China Pulp and Paper

摘　　要：从竹子上分离纯化得到1株产半纤维素酶的菌种,通过出菇实验和ITS序列扩增,研究了其子实体、菌丝形态及分子特征,对菌种进行了分类鉴定。并在静置和振荡培养条件下分析了菌株的酶系组成,该菌主要产半纤维素酶、木素酶和少量纤维素酶。本实验用该菌产生的木聚糖酶协同漆酶/介体进行竹浆生物漂白,酶处理后纸浆白度可达81%以上,黏度下降不多。结果表明,酶处理有利于提高竹浆的可漂性、增加后续漂白中木素的脱除。A fungus producing hemicellulanase was isolated from bamboo. The fungal morphology and ITS sequence were studied. It showed that the fungus was 99% identical to Trametes hirsute. The enzymes produced from the fungus including xylanase, ligninase and cellulanase were analyzed. The activities of xylanase and cellulase in rotating condition were higher than that in static condition. But rotating was in favor of improving laccase and manganese peroxidase activity. Bamboo kraft pulp was biobleached by xylanase and laccase produced from the fungus. The brightness of bamboo kraft pulp was above 81% ISO after enzyme treatment. It indicated that pretreatment with enzyme is in favor of the removal of residue lignin and can improve the bleachability of bamboo kraft pulp.

关 键 词：木聚糖酶 漆酶 序列 培养 生物漂白 

分 类 号：Q55[生物学—生物化学] TS721.1[轻工技术与工程—制浆造纸工程]