CML相关TCR Vα亚家族T细胞的克隆性分布特点  

Analysis of Clonal Expansion of TCR Vα Subfamily T Cells Associated with CML

作　　者：陈思[1] 李扬秋[1] 陈少华[1] 杨力建[1] 卢育洪[1] 

机构地区：[1]暨南大学医学院血液病研究所,广州510632

出　　处：《肿瘤防治研究》2007年第3期168-170,共3页Cancer Research on Prevention and Treatment

基　　金：国家自然科学基金资助项目(30270579);广东省自然科学基金重点资助项目(23001;05103293);血液病学国务院侨办重点学科基金

摘　　要：目的了解慢性粒细胞白血病（CML）患者外周血TCR Vα亚家族T细胞的克隆性分布特点。方法采用RT-PCR扩增10例CML患者外周血的单个核细胞的TCR Vα29个亚家族基因，分析其TCR Vα亚家族的利用情况。PCR产物进一步经基因扫描分析，了解其CDR3长度以判断T细胞的克隆性。结果不同病人外周血中所能检测出的Vα亚家族数量不等（1～21个），以Vα3、Vα4、Vα8、Vα10和Vα14为常见，并在Vα1、Vα2、Vα3、Vα4、Vα5、Vα8、Vα10、Vα12、Vα14、Vα15、Vα16、Vα19、Vα21和Vα23中发现克隆性生长T细胞。结论CML病人外周血T细胞的TCR Vα亚家族选用呈现明显的选择性并出现克隆性T细胞，这可能与机体对白血病相关抗原产生特异性免疫有关，且克隆性增殖Vα亚家族分布以个体特异性为主。Objective To investigate the distribution and clonality of TCR Vα subfamily T cells in patients with chronic myeloid leukemia （CML）. Methods The CDR3 size of TCR Vα 29 subfamily genes was amplified in peripheral blood mononuclear cells from 10 cases with CIVIL using RT-PCR, to observe the usage of TCR Vα repertoire, the PCR products were further analyzed by genescan technique for detection of the CDR3 size, to evaluate clonality of TCR Vα T cells. Results 1-21 Vα subfamily T cells could be identified from different samples in CML cases, predominantly in Vα 3, 4, 8, 10, 14. Oligoclonal （clonal expansion） T cells were identified in Vα 1, 2, 3, 4, 5, 8, 10, 12, 14, 15, 16, 19, 21 and 23 subfamilies. Conclusion The selected usage and clonal expansion of TCR Vα subfamily T cells from peripheral blood could be found in patients with CML, it may be related to CML associated-antigen. Distribution of Vα subfamily clonal expansion displays individual specificity.

关 键 词：TCRVα基因 T细胞克隆性 慢性粒细胞白血病 RT-PCR 基因扫描 

分 类 号：R733.74[医药卫生—肿瘤]