鳗弧菌MVAV6203基因缺失株的生物反应器培养及检测  

Culture and Detection of Vibrio anguillarum MVAV6203 Gene-Deleted Strain

作　　者：杨路[1] 马悦[1] 张元兴[1] 

机构地区：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

出　　处：《水产科学》2007年第4期191-194,共4页Fisheries Science

基　　金：国家863计划资助项目(2003AA622020)

摘　　要：应用摇瓶及30 L生物反应器对鳗弧菌MVAV6203基因缺失株进行培养,通过控制流加速率、改变补料方式及补料基质以优化其培养工艺,并用PCR的方法检测培养所得菌体的aroC基因片段。结果证明:该菌株生长必需添加酪氨酸、色氨酸、苯丙氨酸、对氨基苯甲酸和对羟基苯甲酸;在生物反应器中菌体最大光密度(OD550)达32.0,对应活菌数为4.6×1010cfu/m l;在培养过程中,菌体不发生基因回复突变。Vibrio anguillarum MVAV6203 gene-deleted strain were first incubated in a flask-shaker and 30 liter bioreactor. The process was optimized by controlling flow-rate and changing in supplementary medium. The aroC gene of the cultural strain was detected by PCR method. The results showed that tryptophan, tyrosine, phenylalanine, p-hydroxybenzoic acid and p -aminobenzoic acid had to be added in the medium to make the strain grow. The maximal OD550 in 30 liter bioreactor reached 32.0 and the homologous cell concentration was 4.6×10^10 cfu/ ml. Gene reverse mutation was not observed in the incubation.

关 键 词：鳗弧菌 基因缺失株 培养 氨基酸 

分 类 号：Q936[生物学—微生物学]