大肠癌组织芳香基酰胺酶同工酶的研究  

STUDY ON ARYLAMIDASE ISOENZYME IN THE LARGE BOWEL CARCINOMA

作　　者：葛政举 毛振彪 孟宪镛 倪润洲 施公胜 魏群 

出　　处：《南通医学院学报》1996年第2期173-174,共2页ACTA Academiae Medicinae Nantong

摘　　要：采用自行建立的圆盘等电聚焦电泳将大肠组织中芳香基酰胺酶（ＡＡＤ）分离出１０条区带，从阳极到阴极依次为ＡＡＤⅠ～ＡＡＤⅩ，其中ＡＡＤⅥ主要见于大肠癌组织（７５％）、癌旁组织（５０％），面远癌组织仅９．１％，前二者与后者有显著性差异（ｐ＜０．０５）。大肠癌中ＡＡＤⅥ与Ｄｕｋｅｓ分期和分化程度无相关性（ｐ＞０．０５），其等电点和胎盘型ＡＡＤ（Ｐ－ＡＡＤⅡ）等电点一致（ＰＩ６．３３）。结果表明，ＡＡＤⅥ是大肠癌组织异位表达的证据，可望成为大肠癌及癌前病变的标志物。By means of cylindrical isoelectric focusing,Large bowel tissue arylamidase was separated into ten isoforms, which were named AADⅠ-AADⅩ from anode to cathode,AADⅥ was mainly present in large bowel carcinoma(75.0%)and the tissue adjacent to cancer(50.0%),the tissue remote to cancer (only 9.1%)(P<0.05).There was no correlation among AADⅥ,dukes period and cell classification (p>0.05),AADⅥ was identical with placental type AAD(P-AADⅡ)in isoelectric point (PI 6.33). Clinical research showed that AADⅥ was evident of ectoexpression in large bowel carcinoma tissue and that might be regarded as biochemical marker for diagnosing cancer and precancerous lesions.

关 键 词：芳香基酰胺酶 同工酶 大肠癌 等电聚焦电泳 

分 类 号：R735.34[医药卫生—肿瘤]