枯草芽孢杆菌对多环芳烃的降解能力研究  

Study on Characteristics of Bacillus subtilis strainⅡ for the Degradation of Polycyclic Aromatic Hydrocarbons

作　　者：李丽[1] 钟鸣[1] 周启星[2] 李坤[1] 杨铮[1] 姜树坤[1] 

机构地区：[1]沈阳农业大学辽宁省农业生物技术重点实验室,辽宁沈阳110161 [2]中国科学院沈阳应用生态研究所陆地生态过程重点实验室,辽宁沈阳110161

出　　处：《河南农业科学》2007年第4期62-64,71,共4页Journal of Henan Agricultural Sciences

基　　金：国家"863"计划项目(2004AA649060);中国科学院陆地生态过程重点实验室开放课题资助项目;辽宁省自然科学基金项目(20062111)

摘　　要：从长期受多环芳烃污染的土壤中分离出1株菲降解菌——菌Ⅱ,经生理生化及16S rDNA分析鉴定,该菌株为枯草芽孢杆菌。在单基质菲、芘反应体系中,该菌株具有较强的降解能力。菌Ⅱ不但可以在高浓度的多环芳烃存在下生长良好而且对高浓度多环芳烃有较高的降解能力。多环芳烃与重金属Cu2+的加入对多环芳烃降解菌有很大的影响。在Cu2+浓度小于15 mg/L时,菌Ⅱ能在以多环芳烃为唯一碳源的培养基中生长良好,Cu2+浓度过高将导致菌体死亡。Most of Polycyclic aromatic hydrocarbons（PAHs） are carcinogenesis and environmental pollutant. A phenanthrene-degrading strain, strain Ⅱ isolated from polycyclic aromatic hydrocarbons （PAHs）-contaminated soil was studied and the experiment showed that the strain was able to utilize phenanthrene as the sole carbon source. Based on physical-biochemical characteristics arid phylogenetic analysis of 16S rDNA sequence, strain Ⅱ was identified as Bacillus subtilis. Strain Ⅱ was able to utilize phenanthrene or pyrene as the sole carbon source for growth. After 15 days of cultivation, the concentrations of PAHs could approach 0. PAHs and heavy metal Cu^2＋ could influence the growth of strain 11. Strain 11 could grow well in the medium containing PAHs as sole carbon source when concentrations of Cu^2＋ was lower than 15 mg/L; High Cu^2＋ concentrations inhibited the growth of strain Ⅱ and even resulted in its death.

关 键 词：多环芳烃 生物降解 枯草芽孢杆菌 铜离子 

分 类 号：O625.15[理学—有机化学]