高效液相色谱法测定紫花苜蓿青贮中的有机酸  

Determining organic acid in alfalfa silage by HPLC

作　　者：许庆方[1] 玉柱[1] 韩建国[1] 白春生[1] 薛艳林[1] 荀桂荣 

机构地区：[1]中国农业大学动物科技学院 [2]天津饲草饲料工作站,天津300210

出　　处：《草原与草坪》2007年第2期63-65,67,共4页Grassland and Turf

基　　金：国家"948"项目"退化草地综合改良与建设配套技术"(202099);北京市教委共建项目(XK100190552JD100190531);国家科技成果转化项目"苜蓿发酵液裹包青贮技术的转化"(2002710020908);天津市重大农业科技合作项目"紫花苜蓿拉伸膜青贮技术推广应用"(0202020208)

摘　　要：乳酸、乙酸、丙酸、丁酸是紫花苜蓿青贮中的主要有机酸。运用高效液相色谱仪,Shodex KC-811色谱柱,3 mM高氯酸为流动相,流速1 mL/min,进样量5μL,柱温50℃,岛津SPD-10A检测器,检测波长210 nm,对紫花苜蓿青贮浸提液的乳酸、乙酸、丙酸、丁酸含量同时测定,有机酸分离效果理想,精密度高,回收率好。运用该方法测定数据真实可靠,操作简单。Lactic acid,acetic acid,propionic acid,and butyric acid content in alfalfa silage juices were determined simultaneously by using HPLC（Shodex KC-811 column,3 mM perchloric acid as mobile phase,1 mL/min flow rate,L sampling size, column temperature at 50 ℃ ,Shimadzu SPD-10A detector,and wave length at 210 nm）. The results showed that the organic acids were separated well and the sensitivity and recovery were high; the value were reliable;and the operation were easy.

关 键 词：高效液相色谱仪 苜蓿青贮 有机酸 

分 类 号：O657.7[理学—分析化学] S816.3[理学—化学]