人舌鳞状细胞癌耐放疗细胞系的构建及多药耐药基因和蛋白的表达  

A STUDY OF ESTABLISHMENT OF RADIATION-RESISTANT HUMAN TONGUE SQUAMOUS CARCINOMA CELL LINE AND ITS EXPRESSIONS OF MULTIDRUG-RESISTANT GENE AND PROTEIN

作　　者：韦舜[1] 陈海波[2] 曹 于大海[2] 

机构地区：[1]柳州医学高等专科学校 [2]广西医科大学附属口腔医院,南宁530021

出　　处：《广西医科大学学报》2007年第1期11-13,共3页Journal of Guangxi Medical University

基　　金：国家自然科学基金资助项目(No30060083);广西医科大学博士启动基金(2001)

摘　　要：目的:建立人舌鳞状细胞癌耐放疗细胞系并检测多药耐药基因和蛋白的表达。方法:利用人舌鳞癌细胞株Tca8113,采用单次剂量1Gy,每周2次的60Co照射,诱导其耐放疗性,建立一株耐放疗的人舌鳞癌细胞系。并用免疫组化以及RT-PCR检测多药耐药基因的表达。结果:耐放疗的Tca8113细胞最大耐受剂量为20Gy,多药耐药基因和蛋白表达水平明显增高。结论:成功建立耐放疗细胞系,为口腔癌放疗耐药研究提供了细胞模型。Objeetive： To establish a radiation-resistant human tongue squamous carcinoma cell line and to detect the expression of multidrug-resistant gene（MDR1） and protein （P-gp）. Methods： The Tea8113 cell line was exposed to the 60 Co irradiation, the dosage of which was 1 Gy for two weeks. MDR1 was detected by reverse transcriptase-polymerase chain reaction （RT-PCR） and P-gp by immunohistochemistry. Result： A radiation-resistant Tea8113 cell line was established, and the highest radiation dosage was 20 Gy. The expressions of MDR1 mRNA and P-gp increased significantly. Conelusion.. Irradiation induces a multidrugresistant phenotype in Tea8113 cell line, which provides an ideal model for the study on radiation resistance.

关 键 词：舌鳞癌 细胞系 放疗耐药性 

分 类 号：R730.5[医药卫生—肿瘤]