酪氨酸蛋白激酶受体EphA2及其配体EFNA1在23种肿瘤细胞系中的表达及其意义  

EphA2/EFNA1 expression in 23 human tumor cell lines and its significance

作　　者：赵瑞皎[1] 吴向华[2] 石必枝[1] 王华茂[1] 李宗海[1] 

机构地区：[1]上海市肿瘤研究所癌基因及相关基因国家重点实验室 [2]复旦大学附属肿瘤医院化疗科

出　　处：《中国癌症杂志》2007年第4期320-323,共4页China Oncology

摘　　要：背景与目的:酪氨酸蛋白激酶受体EphA2及其配体EFNA1可能在肿瘤的发生发展过程中起着重要的作用。本研究探讨EphA2/EFNA1在不同肿瘤细胞中的表达及其意义。方法:用RT-PCR方法,检测了23种人类肿瘤细胞系中EphA2/EFNA1在mRNA水平上的表达。并用Western blot方法检测了EphA2蛋白在不同肿瘤细胞系中的表达水平。结果:RT-PCR结果显示,除白血病细胞K562和HL60,以及视网膜母细胞瘤RB几乎检测不到EphA2/EFNA1外,EphA2/EFNA1高表达于肝癌、肺癌、卵巢癌、宫颈癌、前列腺癌、胃腺癌、脑胶质瘤、膀胱癌、成骨肉瘤及恶性黑色素瘤等20种细胞系中。Western blot结果显示,除K562外,EphA2蛋白在不同肿瘤细胞系中均有表达。结论:EphA2/EFNA1高表达于人类多种肿瘤细胞中,并且EFNA1的表达水平相对低于EphA2的表达。E-phA2/EFNA1可作为一个较为广谱的肿瘤标记,并有可能成为肿瘤治疗的靶点。Background and purpose： Tyrosine kinase receptor-EphA2 and its ligand EFNAI have been implicated in many situations leading to carcinogenesis. We investigated the expression the expression levels of EphA2 and EFNA1 in human tumor cell lines. Methods： The expression of EphA2 and EFNA1 was examined using reverse transcription-polymerase chain reaction （RT-PCR） in 23 human tumor cell lines. The expression of EphA2 protein in a number of human tumor cell lines was also examined by Western blot. Results： EphA2/EFNA1 were highly expressed in liver, lung,ovary,cervix,cell prostate, stomach, brain, bladder, bone tumors and melanoma, but it was poorly detected in leukemia and retinoblastoma cell lines. Conclusions： EphA2/EFNA1 are both expressed in human various tumor cell lines, and the expression levels of EFNAI is relatively lower than those of EphA2. EphA2/EFNA1 may be novel tumor markers, and serve as a potential new target for cancer therapeutics.

关 键 词：EphA2受体 EFNA1配体 肿瘤标记 肿瘤细胞系 

分 类 号：R363[医药卫生—病理学]