睫状神经营养因子突变体的设计及活性分析  

The Design and Bioactivity Analysis of CNTF Mutants

作　　者：毕华[1] 岳俊杰[2] 袁力勇[1] 饶春明[1] 陈惠鹏[2] 王军志[1] 

机构地区：[1]中国药品生物制品检定所生化室,北京100050 [2]军事医学科学院生物工程研究所,北京100071

出　　处：《生物化学与生物物理进展》2007年第4期408-411,共4页Progress In Biochemistry and Biophysics

基　　金：国家高技术研究发展计划资助项目(2003AA2Z3480)~~

摘　　要：利用计算机分子模拟系统模建了CNTFRα的三维结构,并对CNTF与CNTFRα结合的关键部位进行分析,同时综合了同源比较的结果及CNTF分子自身的特点,设计了2个CNTF的突变体A和B.表达纯化后得到目的蛋白,TF-1细胞增殖实验的结果表明,2个突变体蛋白的比活性均达到106U/mg的水平,且A的活性大于B的活性.上述结果初步表明设计的合理性,为其进一步的开发和应用奠定了基础.In order to present clues to find safer and more efficient ciliary neurotrophic factor（CNTF）, computer molecular modeling system was used to gain the three-dimension structure of CNTFRα.Then through analysing the CNTF/CNTFRα crucial sites to its bioactivity combined with the homologous sequences comparison and CNTF molecular characters, two CNTF mutants were designed. The mutants＇ bioactivity were tested by the experiment of TF-1 proliferation. The two mutants＇ bioactivity reached 10^6 U/mg level after expression and purification and the bioactivity of A is higher than that of B. The results proved primarily that the design was reasonable and provided premise and basement for CNTF＇s development and application.

关 键 词：计算机分子模拟 表达 纯化 睫状神经营养因子突变体 

分 类 号：Q71[生物学—分子生物学]