文冠果壳提取物对β-淀粉样蛋白致动物学习记忆障碍的改善作用  

Improving effects of extract of the husk of Xanthoceras sorbifolia Bunge on amyloid beta protein-induced learning and memory impairmant in aninal models

作　　者：纪雪飞[1] 刘新霞[2] 吴喆[1] 杨柏珍[3] 王力华[3] 邹莉波[1] 

机构地区：[1]沈阳药科大学药学院,辽宁沈阳110016 [2]河北大学医学部,河北保定071000 [3]中国科学院沈阳应用生态研究所,辽宁沈阳110016

出　　处：《沈阳药科大学学报》2007年第4期232-237,253,共7页Journal of Shenyang Pharmaceutical University

摘　　要：目的研究文冠果壳乙醇提取物对动物学习记忆障碍的改善作用并探讨其可能的作用机制。方法采用水迷宫法、避暗法测定小鼠的学习记忆能力;用Y迷宫法及Morris水迷宫法测试大鼠的学习记忆能力,显微镜下观察海马组织形态学改变。结果文冠果壳乙醇提取物显著减少侧脑室注射β-淀粉样蛋白(25-35)(Aβ(25-35))致记忆障碍小鼠水迷宫实验的逃避潜伏期和避暗实验的错误次数,延长避暗潜伏期;文冠果壳乙醇提取物显著提高D-半乳糖合用Aβ(25-35)致记忆障碍大鼠Y迷宫测试中正确反应率,显著缩短Morris水迷宫测试中的潜伏期及游泳路程;抑制海马神经元的变性及脱落。结论文冠果壳乙醇提取物对Aβ(25-35)致鼠学习记忆障碍有显著的改善作用,其作用机制可能与对抗Aβ(25-35)的毒性有关。Objective To explore the improving effects of ethanol extract of the husk of Xanthoceras sorbifolia Bunge（EXB）on learning and memory in model with Alzheimer＇s disease and preliminarily investigate the mechanism. Methods Water maze task and step-through test were performed to observe the learning and memory ability of mice. Behavioral changes in rats were evaluated using Y maze and Morris water maze task. The alterations in hippocampus morphology were assessed using microscope. Results The escape latency in water maze task and the numbers of error in step-through test were markedly decreased and the right reflect in water maze task and the escape latency in step-through test were increased in AD mice induced by i. c. v. amylaid beta protein（25-35） （Aβ（25-35））. The escape latency and the swimming distance in working memory test were obviously shortened in Morris water maze. The percent alternation was increased in Y maze task. EXB improved neurodegenerative changes and reduced the death of neurons in hippocampus slices in rats impaired by D-galactose and Afl（25-35）. Conclusions These results indicate that EXB significantly ameliorate learning and memory ability in mice and rats with Alzheimer＇s disease and the mechanism may be partially related fo attenuation of the neurotoxicity of Aβ（25-35）.

关 键 词：文冠果壳 阿尔茨海默病 学习记忆 D-半乳糖 β-淀粉样蛋白(25—35) 

分 类 号：R971[医药卫生—药品]