γ射线诱发人正常肝细胞基因组不稳定性研究  

Genomic instability induced by ^60Co γ ray radiation in normal human liver cells

作　　者：耿晓华[1] 郭鲜花[2] 左雅慧[2] 王小莉[2] 王仲文[3] 

机构地区：[1]山西大学生物技术研究所,太原030006 [2]中国辐射防护研究院 [3]中国原子能科学研究院

出　　处：《中华放射医学与防护杂志》2007年第2期135-138,共4页Chinese Journal of Radiological Medicine and Protection

基　　金：中国核工业集团公司基金资助项目（416050202）

摘　　要：目的探讨电离辐射诱发的基因组不稳定性效应。方法采用^60Co γ射线照射人正常肝细胞，检测克隆形成率和微核发生率，利用单细胞凝胶电泳（SCGE）技术检测DNA损伤情况。照射2、4、6、8和10Gy后传代培养，在40代后各剂量组再次统一照射2Gy，进行辐射损伤的检测。结果首次照射后，克隆形成率随受照剂量的增大而降低。存活细胞经二次照射后，SCGE结果和微核发生率结果表明，首次照射剂量与子代二次照射后的损伤程度存在剂量效应关系。结论γ射线不仅在肝细胞中产生直接的生物效应，而且还可以诱发产生可遗传的基因组不稳定性，使子代细胞中的突变频率增加，表现出滞后的遗传改变。二次事件的放大作用是研究基因组不稳定性的一种较好方法。Objective To explore the genomic instability induced by ^60Co γ rays. Methods The cloning efficiency and micronucleus efficiency of normal human liver cell irradiated by ^60Co γ rays were detected, and the method of single cell gel electrophoresis （SCGE） was carried out to measure DNA chains damage. The fast-growlng cells were divided into different dose-groups and then irradiated by ^60Co γ rays. After 40 populations doubling, the progenies were secondly irradiated with 2 Gy ^60Co γ rays. Results The cloning efficiency decreased with the increase of doses after the initial irradiation. After the survival cells were given second irradiation, both results of SCGE and micronucleus frequency showed that the second damage was correlated with the original irradiation doses. Conclusions ^60Co γ rays can not only induce the immediate biological effects in liver cells, hut also lead to the genomic instability in the descendants that leads to an enhanced frequency of genetic changes occurring among the progeny of the original irradiated cell. The expanding effect of second event helps to study the genomic instability.

关 键 词：基因组不稳定性 滞后效应 二次照射 单细胞凝胶电泳 微核 

分 类 号：R686[医药卫生—骨科学]