低氧/复氧刺激对体外培养的胎鼠脊髓OPCs内NRG-1表达的影响  

CHANGES IN EXPRESSION OF NRG-1 IN THE CULTURED OPCS FROM THE SPINAL CORD OF THE EMBRYONIC RAT AFTER STIMULUS OF HYPOXIA/REOXYGENATION

作　　者：刘惠婷[1] 孙燕[1] 林芮禾[1] 刘英[1] 王劼[1] 李瑞锡[1] 彭裕文[1] 

机构地区：[1]复旦大学上海医学院解剖与组织胚胎学系,上海200032

出　　处：《神经解剖学杂志》2007年第2期169-175,共7页Chinese Journal of Neuroanatomy

基　　金：复旦大学青年基金(No.EXF101369)资助项目

摘　　要：为明确脊髓少突胶质前体细胞(oligodendrocyte progenitor cells,OPCs)内neuregulin-1(NRG-1)的表达情况,并探讨脊髓缺血后OPCs中NRG-1的表达变化,本实验采用胎鼠(E11～13)脊髓神经干细胞定向诱导分化所获得的OPCs为实验材料,用免疫组织化学和Western blot方法,比较观察了正常培养和低氧/复氧培养的OPCs中NRG-1的表达。结果显示:(1)正常培养的OPCs中有基础性NRG-1的表达;(2)经低氧/复氧培养后,OPCs中NRG-1的表达2h内骤降,4h时陡升,表达量显著高于其他各时点,此后较迅速地下降,24h后趋于消失。由此推测,脊髓损伤后,OPCs能在缺血缺氧的较早期高表达NRG-1,这可能对于损伤组织的保护和修复有重要意义。In order to understand the normal expression of neuregulin-1 （ NRG-1 ） in oligodendrocyte progenitor cells （OPCs） and the changes of NRG-1 expression after spinal cord ischemia/reperfusion injury, the cultured OPCs from the neural stem cells of the spinal cord of the embryonic rat （E11-13） were used to determine the expression of the NRG-1 in the normal cultured OPCs and changes in expression of the NRG-1 in the OPCs after an exposure of OPCs to normoxic or hypoxic conditions and then let them reoxygenate by means of immunohistochemistry and Western blot. The results showed that： （ 1 ） The NRG-1 was expressed basically in normal cultured OPCs; （2） The expression of NRG-1 decreased immediately after hypoxia within 2 h, and dramatically increased to reach the highest level at 4 h after reoxygenation, then downregulated again continuously till to the level that was hardly detected at 24 h after reoxygenation. These results suggest that there would be a high expression level of NRG-1 of OPCs in early stage of hypoxia, which may play essential role in protecting and reconsitributing injury tissure after spinal cord ischemia/reperfusion injury.

关 键 词：NEUREGULIN-1 少突胶质前体细胞 低氧 细胞培养 大鼠 

分 类 号：R651.2[医药卫生—外科学]