嗅鞘细胞联合神经干细胞脑内移植改善Parkinson病大鼠行为的观察  

OBSERVATION ON THE EFFECTS OF NEURAL STEM CELLS AND OLFACTORY ENSHEATHING CELLS INTRACEREBRAL CO-GRAFT ON THE BEHAVIOR CHANGES IN THE PARKINSON’S DISEASE RAT

作　　者：曾水林[1] 李升[1] 刘钟[1] 李凤飞[1] 朱建宝[1] 李涛[1] 刘卫国[2] 

机构地区：[1]东南大学神经生物学研究所,南京210009 [2]南京脑科医院,南京210029

出　　处：《神经解剖学杂志》2007年第2期181-185,共5页Chinese Journal of Neuroanatomy

摘　　要：为了观察嗅鞘细胞(OECs)联合神经干细胞(NSCs)脑内移植对Parkinson病(PD)的治疗作用,首先将6-OHDA注射至左侧黑质致密部和腹侧被盖区制备PD大鼠。将成功制备的PD大鼠随机分为5组,即0.9%生理盐水对照组、单纯移植NSCs或OECs组、OECs+NSCs共移植组和PD模型对照组。将培养并传至第3代的NSCs、OECs和OECs+NSCs分别移植到PD大鼠的纹状体内,于移植后7、14、30、60d检测PD大鼠旋转行为变化后处死,取脑做冰冻切片,行BrdU、酪氨酸羟化酶(TH)、p75免疫荧光和TH免疫组织化学检测。移植后21d以内,各组大鼠的旋转行为无明显变化;30d和60d时,单纯移植NSCs或OECs组和OECs+NSCs共移植组与生理盐水和模型对照组相比,旋转行为有较明显的改善(P<0.05),其中OECs+NSCs共移植组的旋转行为改善更为显著。在移植后30d和60d,单纯移植NSCs或OECs组在移植区可见少数TH阳性神经元,而OECs+NSCs共移植组的TH阳性神经元明显多于单纯移植NSCs或OECs组(P<0.05)。上述结果表明:OECs能促进胚鼠中脑来源的NSCs向TH阳性神经元分化;单纯移植NSCs或OECs和OECs+NSCs共移植,均能不同程度地改善PD大鼠的旋转行为,但联合移植优于单纯移植。To observe the effects of the neural stem cells（NSCs） and olfactory ensheathing cells （OECs） intracerebral co-graft on the behavior changes in the rat model of Parkinson＇s disease （PD）. The rat model of the PD was established with injecting 6-OHDA into the substantia nigra compacta part （SNc） and ventral tegmental area （VTA）. The PD rats were divided into 5 groups： 0.9% normal saline （NS） control group, pure NSCs or OECs transplantation group, NSCs ＋ OECs co-graft group and PD model control group. NSCs and OECs derived from rat embryonic midbrain and olfactory bulb were pro/pared by culture. The changes of rotating behavior were measured by comparing with different groups at different time points after transplantation. The survived cells were detected by BrdU, p75, TH/p75 immunofluorescent and TH immunohistocbemical staining, respectively. Compared with control groups, there were no obvious changes during the first 3 weeks after transplantation. But at 30 d and 60 d, the rotation was distinctly improved （ P 〈 0.05 ） in the pure NSCs or OECs transplantation group and NSCs ＋ OECs transplantation group, especially in the later group. The emergence of TH-positive cells was at 14 d after transplantation. TH-positive cells were gradually increased during 30 d and 60 d after transplantation. Comparing with NSCs or OECs group, there were more TH-positive cells in the NSCs ＋ OECs group. Our data suggest that OECs may promote the NSCs derived from rat embryonic mesencephalon to become dopaminergic neurons after intracerebral transplantation.

关 键 词：嗅鞘细胞 神经干细胞 脑移植 PARKINSON病 大鼠 

分 类 号：R742.5[医药卫生—神经病学与精神病学]