大鼠脊神经后根切断后脊髓和背根神经节CGRP的表达变化  

CHANGES IN THE EXPRESSION OF CALCITONIN GENE-RELATED PEPTIDE IN SPINAL CORD AND DORSAL ROOT GANGLION AFTER DORSAL RHIZOTOMY

作　　者：谢乐斯[1] 郑林丰[2] 曾志成[1] 张建伟[1] 余清平[1] 许愿忠[1] 王岐本[3] 

机构地区：[1]中南大学湘雅医学院人体解剖与神经生物学系,长沙410013 [2]长沙民政职业技术学院,长沙410073 [3]湘南学院人体解剖学教研室,郴州423043

出　　处：《神经解剖学杂志》2007年第2期209-213,共5页Chinese Journal of Neuroanatomy

摘　　要：为观察大鼠脊神经后根切断后相应背根神经节(DRG)和脊髓节段CGRP的表达变化,本研究采用25只健康成年SD大鼠随机分为正常对照组、假手术对照组和L4、5后根切断后3d、7d和14d组(n=5),用免疫组织化学方法结合图像分析技术检测各组相应DRG和脊髓节段内CGRP的表达变化。结果如下:后根切断后3d、7d和14d伤侧DRG内CGRP表达较对照组和对侧明显增强;后根切断后3d脊髓后角CGRP免疫阳性纤维减少,7d、14d时进一步减少;后根切断后3d脊髓前角运动神经元内CGRP表达增加,免疫阳性细胞数增多,7d和14d时表达进一步增强。以上结果提示,脊神经后根切断后DRG和脊髓CGRP表达变化呈现一定的时空模式,可能参与了神经损伤后的再生过程。To study the changes of the expression of calcitonin gene-related peptidc （CGRP） in the spinal cord and dorsal root ganglia （DR（s） after dorsal rhizotomy, 25 adult Spraguc-Dawlcy rats were randomly divided into normal group, sham-operated group and L4.5 dorsal rhizotomy group, and the experimental group was further divided into 3 d, 7 d and 14 d groups according to the survival time, respectively. The expression of CGRP in the spinal cord and DRG were detected by immunohistochcmieal staining and image analysis methods. The results showed that： CGRP expression in the ipsilatcral DRG was upregulated at day 3, 7 and 14 after dorsal rhizotomy; CGRP immunopositive fibers and terminals in the spinal dorsal horn decreased at day 3, and further reduced at day 7 and 14 ; The number of CGRP-immunopositive motor neurons as well as CGRP immunorcactivity in ventral horn of spinal cord increased after dorsal rhizotomy. We concluded that the changes of CGRP expression presented a temporo-spatial pattern following dorsal rhizotomy, suggesting that CGBP may participate in the process of degeneration and regeneration after nerve injury.

关 键 词：后根切断 CGRP 脊髓 背根神经节 大鼠 

分 类 号：R361[医药卫生—病理学]