淫羊藿苷对破骨细胞的分化及骨吸收功能的影响  

Effects of icariin on the differentiation and bone-resorption function of osteoclasts

作　　者：张大威[1] 程岩[1] 张金超[2] 王乃利[1] 杨梦甦[3] 姚新生[1] 

机构地区：[1]沈阳药科大学天然药物化学教研室,辽宁沈阳110016 [2]香港城市大学生物及化学系 [3]香港城市大学深圳研究院,深圳518057

出　　处：《中国药理学通报》2007年第4期463-467,共5页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金委员会与香港研究资助局联合基金资助项目(No30418007)

摘　　要：目的研究淫羊藿苷对破骨细胞的分化及骨吸收功能的影响,评价淫羊藿苷对骨质疏松的预防及治疗作用。方法采用1,25-(OH)2D3诱导兔骨髓单核细胞形成破骨细胞样细胞以及原代分离的乳兔成熟破骨细胞与骨片共培养的方法,考察淫羊藿苷对破骨细胞的分化及骨吸收功能的影响。结果浓度为100、50μmol.L-1的淫羊藿苷明显抑制1,25-(OH)2D3诱导兔骨髓单核细胞形成破骨细胞样细胞的数目,当其浓度为100、50、10μmol.L-1时,明显抑制破骨细胞的骨吸收功能,具体表现在吸收陷窝数目及表面积均明显减少。结论淫羊藿苷不仅可以明显抑制破骨细胞的分化,同时具有抑制破骨细胞的骨吸收功能的作用,提示淫羊藿苷具有改善骨吸收功能亢进的潜力。Aim To study the main constituent of total flavonoids from herba epimdii, icariin, on the differentiation and bone-resorption activity of rabbit osteoclasts. Methods Applying 1,25-（OH）2D3 induced osteoclast-like multinucleated cells and isolated mature rabbit osteoclasts cocultured with bone slices, the effects of icariin on the differentiation and bone resorbing activity of osteoclasts were investigated. Results Icariin at the concentration of 100, 50μmol· L^-1 significantly inhibited the formation of TRAP positive osteoclast-like cells in rabbit bone marrow cells induced by 1,25-（OH）2D3. Besides, icariin at the concentration of 100, 50, 10 μmol· L^-1 significantly inhibited the bone-resorbing activity of mature osteoclasts： number and surface area of resorption lacuna were greatly suppressed. Conclusion Icariin not only suppressed the bone-resorbing activity of mature osteoclasts but also inhibited the formation of osteoclast-like muhinucleated cells, which has the potential to be the drug candidate for bone loss.

关 键 词：淫羊藿苷 成熟破骨细胞 破骨细胞样细胞 分化 骨吸收 

分 类 号：R-332[医药卫生] R284.1