银杏内酯对拟AD模型大鼠空间学习记忆能力和脑内ChAT表达的影响  

The effect of ginkgolides on expression of ChAT in the Alzheimers disease-like rat brain

作　　者：李清春[1] 蒋天盛[2] 吕心瑞[1] 潘娅[1] 蒋乃昌[1] 

机构地区：[1]贵阳医学院生理学教研室 [2]贵阳市第一人民医院外科,贵州贵阳550004

出　　处：《中国药理学通报》2007年第4期497-501,共5页Chinese Pharmacological Bulletin

基　　金：贵州省优秀科技教育人才省长基金资助项目〔黔科教办(2001)3号〕

摘　　要：目的探讨银杏内酯对拟AD大鼠学习记忆能力影响的可能机制。方法大鼠海马OA(Okadaic acid)微量注射,银杏内酯灌胃。水迷宫实验检测大鼠学习记忆能力;West-ern blot实验检测大鼠海马烟碱型胆碱能受体的表达;免疫组化观察大鼠脑内ChAT的表达。结果与对照组比较,模型组大鼠学习记忆能力明显下降(P<0.01),烟碱型胆碱能受体表达减少(P<0.05),ChAT免疫阳性神经元减少(P<0.05)。与模型组相比,银杏内酯组大鼠学习记忆能力明显提高(P<0.01),烟碱型胆碱能受体表达增多(P<0.05),ChAT免疫阳性神经元增多(P<0.05)。结论银杏内酯提高拟AD大鼠的学习记忆能力可能与其改善模型鼠胆碱能系统的功能有关。Aim To investgate the mechnism through which ginkgolides affect learning and memory capabilities of the Alzheimers disease-like rats. Methods Okadaic acid（OA） was injected into the CA1 region of the rat hippocampus and the rats were gavaged with ginkgolides. The learning and memory abilities of the rats were assessed through Morris water maze behavioral test, and the expressions of nicotinic acetylcholine receptors and ChAT were observed by Western blotting and immunohistochemistry, respectively. Results Compared with the control rats, the capabilities of learning and memory were lowered significantly （P 〈 0. 01 ）and the expressions of both the nicotinic acetylcholine receptors and the ChAT positive neurons were decreased greatly in the model rats（P 〈0. 05）. In the rats gavaged with ginkgolides, it was found that their learning and memory capabilities were improved significantly（P 〈0. 01 ） and that the expression of either nicotinic aeetylcholine receptors or ChAT positive neurons was increased obviously（P 〈0. 05）, as compared with the model rats. Conclusions Ginkgolides are able to enhance the learning and memory capabilities of the Alzheimer disease-like rats, possibly by improving function of the acetylcholine system in the brain.

关 键 词：银杏内酯 学习记忆 烟碱型胆碱能受体 胆碱乙酰转移酶 

分 类 号：R-332[医药卫生]