胃癌相关基因GCRG213真核表达载体的构建及其对胃癌细胞成瘤性的影响  

Construction of gastric cancer related gene GCRG213 eukaryotic expression vector and it’s effect on gastric cancer cells

作　　者：高利利[1] 吴本俨[1] 王孟薇[1] 战大伟[2] 黄海力[1] 伍银桥[1] 尤纬缔[1] 王卫华[1] 

机构地区：[1]解放军总医院南楼消化科,北京100853 [2]军事医学科学院实验动物中心,北京100850

出　　处：《军医进修学院学报》2007年第2期105-107,共3页Academic Journal of Pla Postgraduate Medical School

基　　金：国家自然科学基金资助项目(20370635)

摘　　要：目的:研究胃癌相关基因GCRG213正义、反义转染对胃癌细胞MKN45成瘤性的影响。方法:采用半定量RT-PCR及Western免疫印迹法比较转染不同质粒的MKN45细胞中GCRG213在mRNA和蛋白质水平上的表达差异。选取稳定转染不同质粒的MKN45细胞,绘制细胞生长曲线,平板克隆形成实验、裸鼠移植瘤实验分析转染细胞成瘤性。结果:平板克隆形成实验显示转染了GCRG213正向克隆的MKN45细胞其细胞克隆形成数量高于转染空载体的细胞,而转染了GCRG213反向克隆的MKN45细胞其细胞克隆形成数量低于转染空载体的细胞。在裸鼠体内进行的转染细胞的成瘤性实验可见,转染了GCRG213正向克隆的MKN45细胞在裸鼠体内成瘤性高于转染空载体的细胞,而转染了GCRG213反向克隆的MKN45细胞在裸鼠体内成瘤性低于转染空载体的细胞。结论:胃癌相关基因GCRG213可促进肿瘤细胞的生长,促进肿瘤细胞的成瘤性,GCRG213反义转染可抑制肿瘤细胞的生长,抑制肿瘤细胞的成瘤性。Objctive：To investigate the effect of gene GCRG213 transfection （sense, antisense） on gastric cancer cell MKN45. Methods： Expression of GCRG213 was detected with semi-quantitative RT-PCR and Western Blot. The growth graph was protracted by the methods of cell counting. The clone formation rate in plate and in nude mice was calculated by the oncogenesis characters of transfected cells. Results： In vitro, the average clone formation rate increased in the cells transfected with pcDNA3.1-（ sense） compared with those transfected with vector, while the average clone formation rate decreased in the cells transfected with pcDNA3.1-（antisense）. The time of oncogenesis of pcDNA3.1-（ sense） transducted cells in nude mice was shorter and the tumor nodes were bigger. The time of oncogenesis of pcDNA3.1-（antisense） transducted cells in nude mice was prolonged and the tumor nodes were smaller. Conclusion： GCRG213 promotes tumor cell growth and induced the cells＇ malignancy in vitro and vivo.

关 键 词：胃肿瘤 胃癌相关基因GCRG213 真核表达 基因 转染 

分 类 号：R735.2[医药卫生—肿瘤]