ID4基因表达谱的生物信息学分析  

Analysis of ID4 gene expression series with bioinformatics

作　　者：卢学春[1] 楼方定[1] 朱宏丽[2] 范辉[2] 窦立萍[1] 杨波[3] 康慧媛[1] 于力[1] 

机构地区：[1]解放军总医院血液科,北京100853 [2]解放军总医院南楼血液科,北京100853 [3]吉林大学第一临床医学院,吉林长春130021

出　　处：《军医进修学院学报》2007年第2期140-142,共3页Academic Journal of Pla Postgraduate Medical School

基　　金：国家973重点基金资助项目(2005CB522400);国家自然科学基金资助项目(30572108)

摘　　要：目的:分析ID4基因在正常组织细胞和癌变组织细胞中的表达情况,为研究该基因的表达调控机制奠定基础。方法:采用生物信息学方法,利用开放性的数据库包括Gene expression omnibus(GEO)、Affymetrix基因芯片表达数据库、人类基因组数据库(GeneBank)、基因表达数据库SAGE、GeneCard、InterPro、ProtoNet、UniProt和BLOCKS。结果:ID4基因在多种神经系统肿瘤细胞中的表达高于正常脑组织(209/3,148/3,123/3,72/3,64/3,45/3);而甲状腺滤泡癌的表达要低于正常甲状腺组织(35/172);正常乳腺组织要明显低于转移乳腺癌和原发乳腺癌的表达(10/36,10/34);正常肺组织的表达高于肺腺癌(11/4)。此外,ID4基因在正常淋巴结、胚胎干细胞等细胞中也有表达。结论:生物信息学可以用来方便快捷地分析基因的表达谱;ID4基因的表达具有组织特异性。Objective：To explore the expression series of ID4 gene in normal and malignant tissues or cells as a primary work for studying the regulation mechanism of ID4 gene. Methods： Bioinformatics and free open data-set such as GeneBank, SAGE, GeneCard, GEO, InterPro, ProtoNet, UniProt and BLOCKS were used to analyze the expression series of ID4 mRNA and ID4 protein. Results： ID4 gene had higher expression in a lot of malignant neuro-tissues and cells than in normal brain tissues （209/3, 148/3, 123/3, 72/3, 54/3, 45/3 ） ; higher in normal thyroid cells than in follicular thyroid carcinoma cells （ 172/35 ）, in metastasis and in situ breast carcinoma higher than normal breast （34/10, 36/10） , in normal lung tissues than lung adenocarcinoma（11/4）. Further more, ID4 gene had expression in normal bone marrow, lymphocyte and embryonic stem cell normal cell. Conclusion：Bioinformatics can be used freely to analyze a gene＇s expression series. ID4 gene has different expression in different tissues.

关 键 词：ID4基因 计算生物学 基因表达 

分 类 号：R331[医药卫生—人体生理学]