水稻黄叶标记不育系的诱变选育及其应用  

THE BREEDING OF A RICE XANTHA LEAF MARKER CMS LINE BY GAMMA RAYS MUTATION

作　　者：沈圣泉[1] 周祥胜[1] 吴殿星[1] 舒小丽[1] 叶红霞[1] 舒庆尧[1] 

机构地区：[1]浙江大学农业部核农学重点实验室,浙江杭州310029

出　　处：《核农学报》2007年第2期107-110,123,共5页Journal of Nuclear Agricultural Sciences

基　　金：浙江省"8812"计划专项(2004C12020-1);浙江省科技攻关项目(2006C32015)

摘　　要：用300Gyγ射线辐照Ⅱ-32B干种子,在诱变后代筛选到全生育期黄叶突变体WYB,经多代连续回交转育,育成黄叶标记不育系黄玉A(B),并通过了浙江省科技成果鉴定。经考察,1.黄玉A(B)叶色为明显黄色,对生育进程也有显著影响,表现为植株较矮,稻穗较小,粒重较轻,单株产量较低等;2.遗传分析表明,该黄叶突变性状受一对隐性突变基因控制,且表达稳定,不易受环境影响;3.黄玉A的叶色突变对所配杂种无不良影响,表现为配合力强,所配组合“黄优C23”在金华市区试中产量位居首位,显示出良好的应用前景;4.应用该黄叶标记进行苗期纯度鉴定,不但方法简便、快速,其结果也与常用的国标法(GB/T3543.5-1995)鉴定结果相吻合。A rice mutant WYB, which exhibited xantha leaf during whole growing period, was isolated from Ⅱ-32B with gamma ray irradiation. The noVei CMS line and its maintainer line with the xantha leaf marker, Huangyu A （B）, were developed after a series of backcrosses, and were appraised by the Department of Science and Technology, Zhejiang Province. It suggested that the Huangyu A（B） showed visible xantha leaf marker and different agronomic characteristics, such as lower plant height, smaller panicle, lighter grain weight, lower yield per plant etc. Genetical analysis demonstrated that the xantha leaf mutation was controlled by a single recessive locus, the xantha leaf color was stable at different environment. The leaf mutant had not negative affect on its hybrid offspring. However, the Huangyu A showed higher combining ability. The novel combination Huangyou C23（ Huangyu A/Chihui 23） harvested the highest yield at Jinhua hybrid rice regional test, showed high potential in rice production. By use of the xantha leaf marker, the varietal purity of Huangyu A and its hybrids could be easily and quickly identified at seedling stage. And the purity results were highly consistent with that evaluated in field plot testing at maturity period according to national standard GB/T 3543.5-1995.

关 键 词：水稻 不育系 γ诱变 突变体 叶色标记 

分 类 号：S511[农业科学—作物学]