小鼠皮肤切创后calpain 1和calpain 2的表达  

Expression of Calpain 1 and Calpain 2 during the Skin Incised Wound Healing in Mice

作　　者：张巍[1,2] 官大威[1] 赵锐[1] 张建军[1] 

机构地区：[1]中国医科大学法医学院法医病理教研室 [2]沈阳市公安局交警支队,辽宁沈阳110003

出　　处：《法医学杂志》2007年第2期81-83,F0002,共4页Journal of Forensic Medicine

基　　金：国家自然科学基金资助项目(30271347);教育部留学归国基金资助项目(教外留司[2002]367)

摘　　要：目的观察小鼠皮肤切创愈合过程中calpain1和calpain2表达变化。方法应用免疫组织化学染色技术检测小鼠皮肤切创后calpain1和calpain2的表达。结果对照组的calpain1和calpain2均有表达,伤后1d calpain1阳性细胞率显著增加,达最大值。伤后3d阳性细胞比率显著下降。伤后5d阳性细胞比率再次显著增加,以后逐渐下降。伤后1d calpain2阳性细胞比率显著增加,伤后3d阳性细胞比率显著下降,达最低值。伤后5d阳性细胞比率再次显著增加,达最大值。伤后7d至10d,阳性细胞比率逐渐下降。结论小鼠皮肤切创后,calpain1和calpain2的表达随愈合时间而呈现双峰式变化。Objective To investigate ,the expressions of calpainl and calpain2 during the skin incised wound healing in mice. Methods Expression of calpainl and calpain2 was evaluated by immunohistochemical method. Results Both calpainl and calpain2 were expressed in the skin cells in the control groups. The calpainl positive cells were remarkably increased and reached the maximal level in day 1 after skin incised wound, decreased after day 3, markedly increased again in day 5, and then gradually decreased from day 7 to 14; the calpain2 positive cells were significantly increased in day 1 and decreased to the minimum in day 3, markedly increased again in day 5, and then gradually decreased from day 7 to 10. Conclusion The changes of calpainl and calpain2 expressions appear to be bimodal after incised wound of skin in mice.

关 键 词：CALPAIN 皮肤切创 损伤时间 法医病理学 

分 类 号：R394[医药卫生—医学遗传学] D919[医药卫生—基础医学]