血液中乙醇保存稳定性研究  

Research of on the Stability of Ethanol in Preservation of Ethanol in Blood

作　　者：马栋[1] 卓先义[1] 卜俊[1] 向平[1] 沈保华[1] 

机构地区：[1]司法部司法鉴定科学技术研究所,上海200063

出　　处：《法医学杂志》2007年第2期117-119,共3页Journal of Forensic Medicine

摘　　要：目的确定血液中乙醇最佳保存条件,探讨影响血液中乙醇含量稳定性的主要因素。方法对血液保存的温度(-20、4、20℃)、防腐剂(NaF、无防腐剂、Na2O2)、储存容器中空气所占比例(0%、25%、50%)和血醇质量浓度(0.2、0.8、2.0mg/mL)四个因素采用正交试验L9(34)方法分组,样本采用顶空气相色谱法进行测定,测定结果采用方差分析进行讨论。结果在20℃保存且不加入防腐剂的两组样本中血醇浓度变化明显,其余变化不明显。结论血液样本在4℃、储存容器中空气比例为50%和加防腐剂(NaF)的条件下保存,稳定性最佳;四个影响因素中温度为影响血液中乙醇含量稳定性的主要因素。Objective To focus on the main factors that may influence the stability of ethanol in blood then try to establish the best conditions for preserving ethanol in blood. Methods Four crucial parameters, including temperature （-20, 4, 20 ℃）, preservative （NaF, No preservative, Na202）, percentage of air chamber in container （0%, 25%, 50%）, and ethanol concentration in blood （0.2, 0.8, 2.0mg/mL） were investigated. Blood samples were stored under different conditions designed by orthogonal design L9 （34）method. Quantification of ethanol in blood was carried out by headspace gas chromatography with FID detector. The results were statistically evaluated by analysis of variance. Result Significant changes of ethanol concentrations were observed in the samples stored in 20℃ without any reserveative, while there were no obvious changes in the samples under other storage conditions. Conclusion The best condition for keeping ethanol in blood stable is 4℃ with preservative and with 50% of air chamber in container. The temperature is main effective factor in the four factors.

关 键 词：血液 乙醇 顶空气相色谱法 稳定性 正交试验 保存条件 司法鉴定 法医毒物分析 

分 类 号：D919.4[医药卫生—法医学]