多环芳烃高效降解菌的筛选  

Screening of Immobilized Microorganism Strains Capable of Degrading Polyaromatic Hydrocarbons(PAHs)

作　　者：王新[1] 李培军[1] 巩宗强[1] 张辉[1] V.A.Verkhozina 

机构地区：[1]中国科学院沈阳应用生态研究所 [2]俄罗斯科学院西伯利亚分院闽诺格拉多夫地质研究所,伊尔库茨克664033

出　　处：《农业环境科学学报》2007年第B03期92-96,共5页Journal of Agro-Environment Science

基　　金：国家重点基础研究发展计划973项目(2004CB418506);国家重点基金项目(20337010);中俄自然资源与生态环境联合研究中心基金资助项目

摘　　要：以多环芳烃芘和苯并（a）芘为供试物，对多株土著菌和引进菌同时进行筛选试验。结果表明，引进菌经过驯化后对芘和苯并（a）芘都具有一定的降解能力，降解率在30％～80％，通过SPSS数理统计分析软件对数据进行处理后得出，引进细菌B61、B67、M-B和引进真菌Y219、Y220、M-Y作为固定化包埋的菌种；土著菌对芘和苯并（a）芘的降解率可达40％-95％，经过筛选后确定，土著细菌B02、B07、B09和土著真菌F02、F05、F06作为固定化包埋的菌种。通过试验对上述各菌进行了生长曲线的测定，细菌和酵母菌的对数生长期是5-20h，真菌的对数生长期是10-55h，这为固定化微生物提供了一定的前提条件。Both pyrene and benzo（a） pyrene as experiment materials, multi-genotype indigenous junzhu and introduced strains were simultaneously screened in our lab. The experiment results showed that the introduced strains of after-domestication were capable of degrading ability towards pyrene and benzo（ a）pyrene, respectively. The degradation rates were in a range between 30% and 80%. According to our variation analysis by SPSS software, it inferred that the introduced strains Brl, B67, M-B and yeast Y219, Y220, M-Y, respectively, were immobilized strains. Degradation rates of indigenous strains to pyrene and benzo（a） pyrene were in a range between 40% and 95% , after screening , while indigenous strains B02, B07, B09 and fungi F02, F05, F06 were immobilized strains. It was also found that the growth curve of the above strains for the period of logarithm growth of bacteria was 8-20 h, and that of fungi was 5-55 h, making it possible to provide precondition of immobilized microorganisms.

关 键 词：土著菌 引进菌 筛选 对数生长期 

分 类 号：X172[环境科学与工程—环境科学]