直肠黏膜下注射福尔马林复制的新型内脏炎症痛模型  

Inflammation of the rectum wall induced by formalin as a new model of inflammatory visceral pain

作　　者：张颜波[1] 杨明峰[1] 岳伟[1] 张伟[2] 牛敬忠[1] 吕国蔚[3] 

机构地区：[1]泰山医学院附属医院,山东泰安271000 [2]菏泽医学高等专科学校,山东菏泽274030 [3]首都医科大学神经生物系,北京100054

出　　处：《泰山医学院学报》2006年第7期643-645,共3页Journal of Taishan Medical College

摘　　要：目的为研究内脏炎症痛提供新型动物实验模型。方法选用成年健康的Wistar大鼠,随机分为乙醚麻醉组(A)、直肠插入窥器组(B)、直肠黏膜下注射生理盐水组(C)和福尔马林组(D)。大鼠持续乙醚麻醉下,分别在距离肛门约3.5cm用针长8cm的1ml注射器于直肠粘膜下注射100μl的生理盐水和5%的福尔马林。撤去麻醉后行行为学观察和注射福尔马林处直肠HE染色。结果A、B组大鼠无疼痛反应;C组大鼠在注射生理盐水后60min内表现轻微的疼痛反应;D组大鼠表现出明显的内脏痛行为反应,大鼠在注射福尔马林后30min时达到疼痛评分的最大值,从注射福尔马林后45min至120min,疼痛评分逐渐降低,与C组相比有显著性差异(P<0.01)。HE染色发现A、B和C组大鼠直肠无明显炎症反应,而D组有明显的炎症反应。结论大鼠直肠黏膜下注射福尔马林可以复制内脏炎症痛模型;疼痛分数能客观反映内脏疼痛程度,可作为量化指标。Objective： To provide a new model of inflammatory visceral pain for the research of inflammatory visceral pain. Methods： Adult healthy Wistar rats were divided into 4 groups randomly： A： group of anesthesia; B： group of culoacope insertion ; C ： group of injection of saline into the rectum wall; D： group of injection of formalin. The rats were anesthetized with diethyl ether continuously. A coloscope was inserted through the anus. Under visual control, using an 8 -cm -long needie at about 35ram from the anal margin, 100μl saline and 5% formalin was injected in the submucosa of rectum. Pain scores and HE staining were observed after anesthesia. Results ： Pain behavior was not seen in group A and B. There was light pain behavior in group C in the former 60min after injection of saline. Pain behavior was obvionsly seen in group D and pain scores reached peak at 30min time point after formalin injection. There is no inflammatory reaction in group A, B and C, but obviously in group D. There is significantly difference between group D and C（P 〈0.01 ）. Conclusions： A model of visceral inflammation pain can be produced by rectum submucosal injection of formalin ; Pain scores can objectively evaluate the degrees of visceral pain as a quantitating index.

关 键 词：大鼠 直肠 福尔马林 内脏 疼痛 

分 类 号：R-332[医药卫生]