脑出血后血肿周围神经元Cyt-c与Caspase-3的表达  

Rreleasing of Cyt-c From Neuronal Mitochondria and Activation of Caspase3 in Cytoplasm After Intracerebral Hemorrhage in Rat Models

作　　者：熊新贵[1] 梁清华[1] 包太成[1] 陈疆[2] 谢映[1] 王娟[1] 蒋勇前[1] 刘晓春[1] 

机构地区：[1]中南大学湘雅医院中西医结合研究所,湖南长沙410008 [2]中南大学湘雅医院远程医学中心

出　　处：《实用预防医学》2007年第2期280-282,共3页Practical Preventive Medicine

基　　金：湖南省自然科学基金(01JJY2024);省科技基金(1013-50)

摘　　要：目的观察大鼠脑出血后血肿周围神经元线粒体内细胞色素C(Cyt-c)与活化Caspase-3的表达情况,以探讨脑出血后神经元损伤的机制。方法用Ⅶ型胶原酶脑内立体定位注射诱导大鼠脑出血模型,免疫组化检测神经元线粒体内Cyt-C的释放与血肿周围活化Caspase-3的表达情况。结果大鼠脑出血后血肿周围神经元于6 h即有Cyt-C释放,出现胞浆染色的阳性细胞;1 d阳性细胞表达最多;3 d阳性细胞数减少;5 d阳性细胞继续减少,与假手术组比较差异明显(P<0.01);血肿周围于6 h出现Caspase-3阳性表达细胞,1 d阳性细胞计数达高峰,3 d出现轻度下降,5 d明显下降。与假手术组比较差异明显(P<0.01)。结论脑出血后血肿周围神经元Cyt-c与活化Caspase-3表达明显上调,Cyt-c与Caspase-3在脑出血后神经细胞凋亡中起重要调控作用。Objective To investigate the impaired mechanism n neurons around the haematoma after cerebral hemorrhage through observing the releasing of Cyt- c（Cytochrome c） from mitochondria of neurons around the haematoma and the information of activated Caspase - 3 （cysteinyl aspartate specific proteinase- 3） in cytoplasm. Methods Ⅻ type collagenase was stereotaxically injected into the globus pallidus of rats to establish the ICH model. Immunohistochemical method was used to exam the expression of Cyt- C and activated Caspase - 3 after ICH in rat at different time points. Results Cyt- c was began to release in the neuron around the hematoma after ICH in rat at 6 hours after operation, there were some positive cells stained in the cytoplasm around the hematoma; 1 day thereafter, the cytoplasm was stained strongly, the cell shape became abnormal, and the number of positive cells reached the peak; and at the 3^rd day, the staining began to fade, the positive cells decreased; at the 5^th day, the staining continually reduce and the positive cells declined. The activated Caspase - 3 in cytoplasm appeared around the hematoma at 6 hours after operation, and peaked at the 1^st day, then descended lightly at the 3^rd day, furthered at the 5^th day. As compared with the control group, the difference was significant （P〈 0.01 ）. Conclusion The releasing of Cyt- c from neuronal mitochondria and the activation of Caspase - 3 in cytoplasm appeared quickly around the hematoma after ICH and these results suggest that they play impotrant roles in the neuronal apoptosis after ICH.

关 键 词：脑出血 CASPASE-3 Cyt—c 大鼠 动物模型 线粒体细胞色素C 神经元损伤 

分 类 号：R743[医药卫生—神经病学与精神病学]