日本曲霉β-呋喃果糖苷酶基因克隆及在酿酒酵母中的表达  

Cloning and Expression of β-Fructofuranosidase cDNA from Aspergillus japonicus in Saccharomyces cerevisiae

作　　者：王立梅[1] 齐斌[1] 周惠明[2] 

机构地区：[1]常熟理工学院生物与食品工程系,江苏常熟215500 [2]江南大学食品学院,江苏无锡214122

出　　处：《食品与生物技术学报》2007年第2期62-66,共5页Journal of Food Science and Biotechnology

基　　金：国家自然科学基金项目(30471155)

摘　　要：通过反转录PCR法成功地扩增出日本曲霉β-呋喃果糖苷酶基因,基因片断长度为1 917bp,编码638个氨基酸。将所得片断定向克隆到pYX212载体上,并转化至酿酒酵母中,通过酵母菌落PCR检验后,确定该基因在酿酒酵母中获得表达,转化子平均酶活为26.4 U/mg。A β-fructofuranosidase gene of 1 917 bp was cloned from Aspergillus japonicus, and the ORF sequence predicted a protein of 638 amino acids. A expression plasmid pYX212 carrying the gene was under the control of the triose phosphate isomerase gene （TPI） promotor was transformed into Saccharomyces cerevisiae W303-1A, and β-fructofuranosidase gene was successfully expressed in the recombinant strains. The enzyme activity was about 26.4 U/mgo

关 键 词：日本曲霉 Β-呋喃果糖苷酶 CDNA克隆 酿酒酵母 

分 类 号：Q786[生物学—分子生物学]