CYP2D6 C188T单核苷酸多态与肺癌易感性  

Single Nucleotide Polymorphisms of CYP2D6 C188T and Susceptibility to Lung Cancer

作　　者：燕贞[1] 吴拥军[1] 吴逸明[1] 

机构地区：[1]郑州大学公共卫生学院劳动卫生学教研室,河南郑州450001

出　　处：《环境与职业医学》2007年第2期167-169,共3页Journal of Environmental and Occupational Medicine

基　　金：国家自然科学基金资助项目(编号:30571552);河南省中青年骨干教师资助项目;郑州市科技发展计划项目(编号:052SGY33212)

摘　　要：[目的]分析CYP2D6(cytoehromes P450 2D6)基因C188T单核苷酸多态与肺癌风险的关系,探讨肺癌的敏感基因型。[方法]以病例-对照研究方法,采用聚合酶链反应-限制性片段长度多态性方法(PCR-RFLP),检测118例肺癌患者和按性别、年龄频数匹配的118例正常对照者的CYP2D6基因C188T多态,分析各基因型与肺癌易感性的关系。[结果]肺癌组和对照组人群188T等位基因频率分别为53．39％和61.86％,T188／T基因型频率分别为29．66％和42．38％,差异均无统计学意义(P>0．05)。经年龄、性别、吸烟情况调整后非突变基因型T188／T(即野生基因型C188／C和杂合基因型C188／T合并)与肺癌有中等强度的关联OR=1．70(95％CI:1．01～2．92),尤其在鳞癌中关联更明显,OR=2．49(95％CI: 1．07～5．79);对吸烟程度所作分层分析结果表明,在不吸烟者及轻度吸烟者中非T188／T基因型的个体患肺癌的风险显著增高,其调整OR值分别为2．28(95％CI:1．08-4．81)和3．64(95％CI:1.13-11．79);CYP2D6非T188／T基因型与吸烟在肺癌的发生过程中不存在交互作用(x2=1．46,P=0．227)。[结论]T188／T基因型在不吸烟者、轻度吸烟者中及肺鳞癌中可能作为保护因素而降低肺癌的易感性。[ Objective ] To analyze the correlation between the single nucleotide polymorphism of CYP2D6 C188Tand the risk of lung cancer, and explore the sensitive genotype of hmg neoplasm. [ Methods ] A case-control study of 118 lung cancer patients and 118 control subjects（ matched for sex and age ）was carried out to investigate the relationship between C188Tand lung cancer taking by PCR-RFLP to identify the genotype frequencies. [ Results ] The frequency of 188T allele in case and control groups was 53.39% and 61.86% respectively; and that of T188/Tgenotype was 29.66% and 42.38%. The differences of 188T allele and T188/Tgenotype distribution were not statistically significant between cases and controls. Non-T188/T genotype （ including C188/C and C188/T genotype ）was connected with lung cancer moderately and the OR after adjusted by gender, age and smoking was 1.70 （ 95%CI： 1.01～2.92 ）, especially with hmg squamous carcinoma the OR was 2.49（ 95%CI： 1.07～5.79 ）. After stratification analysis according to smoking and adjusted by gender and age it revealed that Non-T188/Tgenotype was associated with significant increased risk in nonsmokers and light-smokers group with OR=2.28（ 95%CI： 1.08～4.81 ）and OR=3.64（ 95%CI： 1.13～11.79 ）respectively. There was no interaction between non-T188/Tgenotype and smoking on susceptibility to lung cancer（χ^2=1.46,P=0.227 ）. [ Conclusion ] T188/T genotype may act as a protective factor in non-smokers or light-smokers subgroups and in hung squamous carcinoma cases.

关 键 词：CYP2D6 基因多态性 肺癌易感性 

分 类 号：R734.2[医药卫生—肿瘤]