高效石油降解菌的筛选及石油污染土壤生物修复特性的研究  

Isolation of petroleum degradation bacteria and its application to bioremediation of petroleum-contaminated soil

作　　者：徐金兰[1] 黄廷林[1] 唐智新[1] 肖洲强[1] 

机构地区：[1]西安建筑科技大学环境与市政工程学院,西安710055

出　　处：《环境科学学报》2007年第4期622-628,共7页Acta Scientiae Circumstantiae

基　　金：国家自然科学基金资助项目(No.50378077)~~

摘　　要：从陕北石油污染土壤中富集分离、优选出7株菌株,并进一步研究了7株菌的生理生化特性.菌株鉴定结果表明,SY21为不动细菌属,SY22为奈瑟氏球菌属,SY23为邻单胞菌属、SY24为黄单胞菌属、SY42为动胶菌属、SY43为黄杆菌属、SY44为假单胞菌属.7株菌的降油试验结果表明,降解8d后,加菌试样的石油烃降解率均达到80%左右,7株菌的石油烃降解速率高于目前已有的报道.接种量越大,石油菌数量越多,石油烃降解率随接种量的增加而提高.采用SY43和SY23菌株对土壤进行生物修复,试验结果表明,投加高效菌株SY43和SY23均可在较短的时间内将土壤中的石油污染物去除,去除率可达88.4%和73.4%,其中菌株SY43的修复效果优于SY23.7 strains of bacteria were isolated from petroleum-contaminated soil in north of Shaanxi province and their physiological characteristics were investigated. Results showed that strain SY21 was Acinetobacter sp. , strain SY22 Neisseria sp. , strain SY23 Plesiomonas sp. , strain SY24Xanthomonas sp. , strain SY42 Azotobacter sp. , strain SY43 Flavobacterium sp. , and strain SY44 Pseudomonas. sp.. Removal efficiency of the bacteria to TPH （ Total Petroleum hydrocarbon） reached about 80% after 8 days＇inoculation, which is higher than that of bacteria reported. For strain SY43 , the more inoculation the higher removal efficiency to TPH achieved. The experiment of application strain SY43 and SY23 to remedy petroleum-contaminated soil indicated that they could remove 88.4% and 73.4% of TPH, respectively within short period. The strain SY43 was more efficient in bioremediation than that of SY23.

关 键 词：高效降油菌 石油污染土壤 接种量 生物修复 

分 类 号：X172[环境科学与工程—环境科学]