抗癌方阻止大鼠肝癌前病灶生长及肝癌形成的研究  

Intervention of Kangaifang on the growth of pre-neoplastic lesions and the development of hepatocellular carcinoma in the rat

作　　者：周小舟[1] 邱光清[2] 贺劲松[1] 周大桥[1] 童光东[1] 郑颖俊[1] 徐韶敏[1] 

机构地区：[1]广州中医药大学附属深圳医院国家中医肝病重点专科,广东深圳518033 [2]广东省药物研究所

出　　处：《中西医结合肝病杂志》2007年第2期92-93,共2页Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases

基　　金：广东省卫生厅;广东省中医药管理局立项课题(No.103096);深圳市科技局立项课题(No.200004091)

摘　　要：目的:观察抗癌方阻止肝癌前细胞增殖和发展的情况,并阐明抗癌方防治肝癌变的作用及机制。方法:观察实验第4周和第40周抗癌方对DEN+2-AAF+PH化学诱导SD大鼠肝癌变模型的影响。结果:抗癌方,①能明显减少癌前病灶数量、缩小癌前病灶平均体积;②能明显减少癌前病灶中增殖细胞核抗原指数;③用药40周后能明显抑制肿瘤的形成和发展;对于肿瘤的数量和平均体积,长期用药较短期用药明显减少。结论:抗癌方通过抑制癌前细胞增殖和可能部分依赖p53独立途径诱导p21表达,直接阻止和延缓肝癌前病变的形成和发展,具有阻止肝癌变和肝癌形成的作用。Objective： To observe the effect of Kangaifang （KAF） preventing hepatocarcinogenesis the mechanism. Methods： The cally induced hepatocarcinogenesis model initiated by diethylnitrosamine （DEN） and promoted by 2-acetylamin- ofluorene （2-AAF） and partial hepateetomy, in which hepatoeellular carcinoma （HCC） arises through pre-neoplastie loci without inflammation or fibrosis. The protocols of KAF administration were started simultaneously with chemical initiation and lasted for either 4 or 40 weeks. Results ： As a result of KAF treatment, ①the numbers and average volume of pre - neoplastic loci were reduced. ②The proliferating cell nuclear antigen index was also reduced in the pre - neoplastic foci in the KAF-treated group. ③ KAF also suppressed the tumor development at 40 weeks after initiation. And in the long-term KAF-treated group, both the tumor number and average tumor size were markedly more reduced than those in the short-term-treated group. Conclusion： It was shown that KAF directly prevented and delayed hepatocarnogenesis through the suppression of pre - neoplasitc cell proliferation and that it may partially depend on p21 induction through a p53-independent pathway.

关 键 词：中药 抗癌方/药效学 肝癌变 大鼠 

分 类 号：R735.7[医药卫生—肿瘤]