Simulation of Flux Distribution in Central Metabolism of Saccharo-myces cerevisiae by Hybridized Genetic Algorithm  

作　　者：张慧敏 姚善泾 

机构地区：[1]Department of Chemical and Biochemical Engineering, Zhejiang University

出　　处：《Chinese Journal of Chemical Engineering》2007年第2期150-156,共7页中国化学工程学报（英文版）

基　　金：Supported by the National Natural Science Foundation of China (No.20276065);the Special Funds for Major State BasicResearch Program of China (973 Program, 2007CB707805).

摘　　要：A scheme of investigating the intracellular metabolic fluxes in central metabolism of Saccharomyces cerevisiae based on isotope model and tracer experiment was developed. The metabolic model applied in this study includes the Embden-Meyerhof-Parnas pathway,the pentose phosphate pathway,the tricarboxylic acid cycle,CO2 anaplerotic reactions,ethanol and acetate formation,and pathways involved in amino acid synthesis. The approach of hybridized genetic algorithm combined with the sequential simplex technique was used to optimize a quadratic error function without the requirement of the information on the partial derivatives. The impact of some key pa-rameters on the algorithm was studied. This approach was proved to be rapid and numerically stable in the analysis of the central metabolism of S.cerevisiae.A scheme of investigating the intracellular metabolic fluxes in central metabolism of Saccharomyces cerevisiae based on isotope model and tracer experiment was developed. The metabolic model applied in this study includes the Embden-Meyerhof-Parnas pathway, the pentose phosphate pathway, the tricarboxylic acid cycle, CO2 anaplerotic reactions, ethanol and acetate formation, and pathways involved in amino acid synthesis. The approach of hybridized genetic algorithm combined with the sequential simplex technique was used to optimize a quadratic error function without the requirement of the information on the partial derivatives. The impact of some key parameters on the algorithm was studied. This approach was proved to be rapid and numerically stable in the analysis of the central metabolism of S.cerevisiae.

关 键 词：Saccharomyces cerevisiae metabolic flux hybridized genetic algorithm 2D NMR central metabolism 

分 类 号：TS262.5[轻工技术与工程—发酵工程]