红霉素致小鼠肝脏毒性效应的基因表达谱变化  

Expression profiles analysis of toxic effect in babl/c mouse liver exposured to erythromycin lactobionate

作　　者：高利宏[1] 敖林[1] 胡冉[1] 刘晋祎[1] 黄明辉[2] 杨梦苏[2] 曹佳[1] 

机构地区：[1]第三军医大学预防医学系卫生毒理教研室,重庆400038 [2]香港城市大学基因组科技应用研究中心,深圳518057

出　　处：《重庆医学》2007年第8期721-723,共3页Chongqing medicine

基　　金：国家杰出人才基金资助项目(3012503);国家自然科学基金资助项目(30200354;30271136);国家973课题分题(2002CB512901)。

摘　　要：目的利用小鼠毒理基因芯片观察红霉素致balb/c小鼠肝脏毒性效应的基因表达谱变化。方法建立红霉素致balb/c小鼠肝毒性效应模型,利用本室构建的小鼠毒理基因芯片检测1、3、7d小鼠肝脏基因表达谱变化,结合层次聚类和生物信息数据库检索对差异表达基因进行初步信息分析。结果各时相组共发现239个差异表达基因,其基本表达模式分为4群,结合功能分析涉及脂肪分解代谢、蛋白质合成与降解、氧化应激、炎症发生、凋亡相关信号转导等多方面机制。结论毒理芯片的检测展示了红霉素致肝脏毒性效应的基因表达谱变化基本轮廓,为进一步阐明其肝毒性机制提供了众多线索。Objective To observer the change on the expression profiles for the toxic effect in babl/c mouse liver acted by erythromycin lactobionate using toxicological microarray. Methods Utilizing the mouse toxicological microarray and animal model of erythromycin lactobionate injuring balb/c mouse liver, which established by ourself, the gene expression profiles on 1,3,7d after drugs treated were observed, and the data were primarily analysed by hierarchical clustering and querying bioinformation databases. Results Two hundred and thirty-nine differential expressed genes were found in several groups, which expressive models were divided into four cluster, and the multiple mechanism associated with fat catabolism, protein synthesis and decompose, oxidation hyperirritability, inflammation, signal transduction about apoptosis, and so on, were suggested by function analysis. Conclusion The elementary figure of gene expression profiles about the toxic effect in liver acted by erythromycin lactobionate was exhibited by toxicological microarray, offering many benefits to elucidate the molecular mechanism of the drug＇s hepatotoxicity.

关 键 词：红霉素 基因表达谱 肝脏 

分 类 号：R365.595[医药卫生—病理学]