莪术醇生物修饰构建的瘤苗治疗胃癌的实验研究  

Treatment effect reaserch on gastric tumor by cancer cell vaccine modified with Curcumol

作　　者：徐立春[1] 许祥裕 陈平[3] 周娟 刘志敏 孙振华 边可君 朱海航[3] 卜平[1] 

机构地区：[1]扬州大学中西医结合研究所,江苏扬州225001 [2]南京军事医学研究所,江苏南京210002 [3]苏北人民医院,江苏扬州225002 [4]不详

出　　处：《实用肿瘤杂志》2007年第2期108-111,共4页Journal of Practical Oncology

基　　金：江苏省医学卫生重大科研课题资助项目(编号K200511)

摘　　要：目的探讨中药莪术的提取物——莪术醇修饰构建的肿瘤细胞疫苗对SGC-7901胃癌的抗瘤效应及联合新城鸡疫病毒(NDV)疫苗的综合效应。方法对SGC-7901胃肿瘤细胞进行系列处理,用莪术醇及NDV对其进行系列生物构建,修饰构建的瘤苗经免疫小鼠(1次/周×3)21天后,接种SGC-7901胃肿瘤细胞,根据设计观察各组抑制肿瘤肺转移、皮下结节形成、生存时间状况及LAK细胞的杀伤效应。结果经莪术醇修饰构建的SGC-7901肿瘤疫苗与对照组相比能明显阻止胃癌细胞的肺转移,显著抑制皮下肿瘤结节形成,明显延长荷瘤鼠的生存时间(P<0.05);用各组免疫接种后长期存活的脾细胞制备的LAK细胞,较同龄未免疫小鼠的脾细胞制备的LAK细胞具有更强的抗瘤效应(P<0.05);由NDV构建的疫苗的抗瘤作用低于莪术醇瘤苗组,莪术醇与NDV联合构建的瘤苗其抗瘤作用未呈现生物放大及相加效应。结论莪术醇修饰构建的SGC-7901新型肿瘤疫苗可以增强胃肿瘤细胞的免疫原性,对胃癌有较好的实验治疗作用。Objective To observe the treatment effect on SGC-7901 by Curcumol modifying tumor cell vaccine,and combine NDV modifying tumor ceil vaccine. Methods After inactivation, the SGC-7901 tumor cells were modified by Curcumol and NVD was vaccinated with the inactivated and modified tumor cell. Then the BALB/c mice were inoculated with SGC-7901. Gastric cancer ceil and treatment effect was observed. Results Curcumol modified SGC-7901 cells effectively induced tumorogenicity of SGC-7901 Curcumol cells was remarkably lowered in vivo and could have good effects on gastric cancer in the BALB/c mice. Treatment effect of Curcumol-modifing cell vaccine is better than NDV group and control group. Conclusion The immunogenenicity of Curcumol-modified SGC-7901 cancer ceil vaccine is increased and the cancer ceil vaccine modified by Curcumol has marked effect against SGC-7901 （gastric cancer） in the BALB/c mice.

关 键 词：胃肿瘤/治疗 癌症疫苗/治疗应用 莪术醇/分离和提纯 癌症疫苗/生物合成 

分 类 号：R735.2[医药卫生—肿瘤]