原发性肝癌组织中Kruppel样因子6(KLF6)的表达及其对肝癌细胞增殖的抑制作用  

Expression of tumor suppressor Kruppel-like factor 6(KLF6) gene in hepatocellular carcinoma and its effect on growth of SMMC-7721 cells

作　　者：潘修成[1] 陈智[1] 陈峰[1] 陈晓红[1] 周承[1] 羊正纲[1] 

机构地区：[1]浙江大学医学院附属第一医院传染病研究所卫生部传染病重点实验室,浙江杭州310003

出　　处：《实用肿瘤杂志》2007年第2期125-129,共5页Journal of Practical Oncology

摘　　要：目的了解Kruppel样因子6（KLF6）基因在原发性肝癌组织中的表达水平及其对肝癌细胞增殖的抑制作用。方法分别以逆转录聚合酶链反应（RT-PCR）和Wstern blot检测原发性肝癌及其癌旁组织KLF6 mRNA及蛋白质的表达水平。构建KLF6真核表达质粒并转染肝癌细胞SMMC-7721,流式细胞仪及MTT试验观察KLF6基因对细胞周期及细胞增殖活性的影响。以Western blot技术检测外源KLF6基因对p21WAF1表达的影响。结果与对应的癌周组织比较,23例原发性肝癌组织中8例（34.8%）KLF6 mRNA,9例（39.1%）KLF6蛋白质水平明显下降。与对照组比较,转染KLF6基因的SMMC-7721细胞G0~G1期所占细胞的比例明显增高,转染KLF6基因SMMC-7721的增殖率明显下降。外源KLF6基因能上调肝癌细胞p21WAF1表达。结论KLF6基因表达水平的下降可能在原发性肝癌发病机制中起到一定作用。上调p21WAF1表达是KLF6基因抑制肝癌细胞增殖的重要途径.Objective To investigate the expression of Kruppel-like factor 6 （KLF6） in hepatocellular carcinoma （HCC） tissues and its inhibitory effect on the growth of SMMC-7721 cells. Methods The expression of KLF6 mRNA and protein in 23 tissue specimens of HCC and adjacent normal tissues by reverse transcription- polymerase chain reaction （RT- PCR） and Western blot respectively. Growth inhibition was analyzed by cell cycle analysis and MTT assay in human HCC cell line SMMC-7721. Expression of p21^WAF1,a possible downstream gene of KLF6,was detected in SMMC-7721 cells transfected with KLF6 gene. Results Compared with corresponding adjacent tissues,expression of KLF6 mRNA and protein was downregulated in 8（34.8%） and 9（39. 1%） HCC specimens respectively. Infection of SMMC-7721 cells with KLF6 gene inhibited cellular proliferation and prolonged G1-S transition by inducing p21^WAF1 expression. Conclusion KLF6 gene suppresses hepatocellular carcinoma cell proliferation,which may be partially associated with the upregulation of p21^WAF1 expression.

关 键 词：癌 肝细胞/病理学 基因 肿瘤抑制 基因表达 转染 增殖细胞核抗原 基因蛋白质p21 

分 类 号：R735.7[医药卫生—肿瘤] R730.2[医药卫生—临床医学]