全反式维甲酸对激素非依赖性前列腺癌连接蛋白43表达的影响  

Effect of all-trans retionic acid on expression of connexin 43 in androgen-independent prostate cancer

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作  者:陈卫国[1] 严春寅[1] 侯建全[1] 李纲[1] 温端改[1] 

机构地区:[1]苏州大学附属第一医院泌尿外科,苏州215006

出  处:《肿瘤》2007年第3期176-178,共3页Tumor

基  金:江苏省科技发展计划基金项目(编号:BS2003013)

摘  要:目的:探讨全反式维甲酸(all-trans retinoic acid,ATRA)对激素非依赖性前列腺癌连接蛋白43(Cx43)表达的影响及其临床价值。方法:应用激素非依赖性前列腺癌细胞PC-3细胞建立荷前列腺癌裸鼠模型,分为ATRA治疗组(n=9)和对照组(n=9),半定量逆转录聚合酶链式反应(RT-PCR)和免疫组织化学法分别检测肿瘤组织中Cx43的表达,并动态观察肿瘤生长抑制情况。结果:与对照组比较,ATRA可以显著抑制肿瘤生长(P<0.05)。RT-PCR检测结果提示,治疗组中Cx43 mRNA表达量为2.03±0.85,明显高于对照组的0.99±0.23(P<0.05)。免疫组织化学法显示对照组肿瘤组织中Cx43蛋白表达微弱;而治疗组中表达量明显升高,以细胞膜上表达为主。结论:激素非依赖性前列腺癌组织中Cx43表达低下,ATRA可以提高其表达而抑制肿瘤的生长。Objective:To investigate the effects and clinical value of all-trans retinoic acid (ATRA) on the expression of connexin (Cx)43 in androgen-independent prostate cancer, Methods:Androgen-independent prostate cancer cell line PC-3 was subcu- taneously transplanted to nude mice. The tumor-bearing nude mice were randomly divided into control group (n=9) and ATRA group (n=9). Cx43 expression was examined with reverse transcriptase-polymerase chain raction (RT-PCR) and immunohisto- chemical method in tumor tissues of both groups. The growth of tumor was observed dynamically, Results: ATRA significantly inhibited the tumor growth compared with control group (P〈0.05), The results of RT-PCR showed that the expression of Cx43 mRNA was 2.03±0.85 in ATRA group which was higher than control group (0.99±0.23, P〈0.05). Immunohistochemical analysis showed that Cx43 protein was abundant and mainly located in membrane in ATRA group, but it was weak in control group. Concision,Androgen-independent prostate cancer cell line PC-3 had weak Cx43 expression, ATRA inhibits the growth of PC-3 cells via enhancing the expression of Cx43.

关 键 词:前列腺肿瘤 维甲酸 连接蛋白43 

分 类 号:R737.25[医药卫生—肿瘤]

 

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