Arresten抑制HUVEC细胞增殖和管腔形成的实验研究  

Arresten inhibited human umbilical vein endothelial cells proliferation and tube formation

作　　者：胡青钢[1] 郑启昌[1] 龙淼云[1] 宋自芳[1] 陈立波[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院肝胆外科,武汉430022

出　　处：《肿瘤》2007年第3期179-180,198,共3页Tumor

基　　金：国家自然科学基金(编号:30271242;30371396)

摘　　要：目的:探讨血管生成抑制因子arresten对人脐静脉内皮细胞HUVEC增殖和体外血管腔形成的影响,了解其抑制血管生成的途径。方法:采用MTT比色法,观察不同浓度的arresten对HUVEC细胞增殖的影响;Matrigel胶体外管腔形成试验检测arresten对HUVEC细胞形成血管的影响。结果:MTT比色法显示,arresten对HUVEC细胞增殖具有抑制作用,随着浓度的增加,arresten对HUVEC细胞增殖抑制作用也增强,但当其浓度增加到800 ng/mL后其增殖抑制率不再提高; arresten浓度为0、400及800 ng/mL时,HUVEC细胞形成的血管腔数分别为17±2、9±1及5±1。结论:Arresten能够抑制HUVEC细胞的增殖,并抑制HUVEC细胞形成血管腔。Objective： To investigate the effect of arresten, an inhibitor of angiogenesis, on human umbilical vein endothelial cell （HUVEC） proliferation and tube formation in vitro and understand its action pathway. Methods： MTT method was used to access the effect of arresten at different concentrations on the proliferation of HUVEC. Capillary formation was detected by Matrigel-plug assay in vitro. Results： MTT assay showed that arresten inhibited the proliferation of HUVEC in a concentration-dependent manner. But the inhibitory rate did not increase when the concentration of arresten reached 800 ng/mL. The tube number was 7±2, 9±1, and 5±1 when the concentration of arresten was at 0, 400, and 800 ng/mL, respectively. Conclusion： Arresten can inhibit HUVEC proliferation and tube formation in vitro.

关 键 词：肿瘤 血管生成抑制剂 ARRESTEN 细胞增殖 

分 类 号：R730.2[医药卫生—肿瘤]