DNA-PKcs和Bcl-2在急性白血病细胞中的表达及相关性研究  

Expressions of DNA-PKcs and Bcl-2 and their relationship in patients with acute leukemia

作　　者：刘殊[1] 冯丹[1] 刘云鹏[2] 侯柯佐[2] 于萍[2] 

机构地区：[1]中国医科大学附属第四医院肿瘤内科,辽宁沈阳110032 [2]中国医科大学附属第一医院肿瘤内科,辽宁沈阳110001

出　　处：《中华肿瘤防治杂志》2007年第6期430-432,共3页Chinese Journal of Cancer Prevention and Treatment

基　　金：辽宁省自然科学基金(962303);辽宁省教育厅基金(20122152)

摘　　要：目的:研究DNA-PKcs和Bcl-2在急性白血病(AL)细胞中的表达及相关性。方法:采用免疫组化SP法检测62名初治AL患者骨髓白血病细胞DNA-PKcs和Bcl-2的表达。结果:DNA-PKcs在对化疗药物耐药患者白血病细胞中的表达率(51.5%)明显高于敏感组(20.7%),P<0.05。Bcl-2在对化疗药物耐药患者白血病细胞中的表达率(84.8%)明显高于敏感组(62.1%),P<0.05。耐药组患者的白血病细胞中DNA-PKcs(+)/Bcl-2(+),DNA-PKcs(+)/Bcl-2(-),DNA-PKcs(-)/Bcl-2(+)和DNA-PKcs(-)/Bcl-2(-)细胞的百分率分别是88.2%,33.3%,44.8%和30.0%,DNA-PKcs(+)/Bcl-2(+)与DNA-PKcs(+)/Bcl-2(-)、DNA-PKcs(-)/Bcl-2(+)和DNA-PKcs(-)/Bcl-2(-)之间差异均有统计学意义(P<0.05,P<0.01,P<0.01)。33名耐药组患者中,DNA-PKcs和Bcl-2的表达无相关性,r=0.171,P>0.05。29名敏感组患者中,DNA-PKcs和Bcl-2的表达呈负相关,r=-0.45,P<0.05。结论:DNA-PKcs可能下调Bcl-2的表达,AL患者的白血病细胞中DNA-PKcs和Bcl-2表达水平增高与临床耐药密切相关。OBJECTIVE： To study the expressions of DNA-PKcs and Bcl-2 and their relationship in acute leukemia （AL） patients. METHODS： The expressions of DNA-PKcs and Bcl-2 in bone marrow mononuelear cells of 62 AL patients were measured by SP immunohistoehemistry method. RESULTS： The positive expression rate of DNA-PKcs in the treatment resistant group（51.5%）was significantly higher than that in the sensitive group（20.7 % ）, P〈0.05. The positive expression rate of Bcl-2 in the treatment resistant group （84.8 %） was significantly higher than that in the sensitive group（62.1%）,P〈 0.05. The percentage of patients with DNA-PKcs （ ＋ ）/Bcl-2 （＋） in the treatment resistant group（88.2% ） was significantly higher than that of ones with DNA-PKcs （＋）/Bcl-2 （-） （33.3%）,DNA-PKcs（-）/Bcl-2（＋）（44.8%） and DNA-PKcs （-）/Bcl-2（- ）（30.0%）, respectively（P〈0.05, P〈0.01, P〈0.01）. In the treatment resistant group, there was no correlation between DNA-PKes and Bcl-2 expression, r= 0. 171, P〉0.05. However, in the treatment sensitive group, negative correlation existed between the expression of DNA-PKes and Bcl-2, r=0.45,P〈0.05. CONCLUSIONS： DNA-PKcs probably down-regulates Bcl-2 in triggering apoptosis in response to se vere DNA damage, and the higher expressions of DNA-PKcs and Bcl-2 are closely correlated with clinical drug resistance.

关 键 词：白血病 急性病 免疫组织化学 

分 类 号：R733.7[医药卫生—肿瘤]