DBA/2J高眼压模型小鼠和正常BL6小鼠眼犬尿氨酸转氨酶-Ⅱ的表达差异  

Difference of kynurenine aminotransferase-Ⅱ in eyes with DBA/2J intraocular hypertension model and normal BL6 mice

作　　者：熊新春[1] 郑红梅[1] 赵战鹰 章艳[1] Sylvia Bolz 

机构地区：[1]华中科技大学同济医学院附属协和医院眼科,武汉430022 [2]湖北省当阳市人民医院眼科,444100 [3]德国Tuebingen大学眼科视觉和神经眼科病理研究室,tuebingen72076

出　　处：《眼科研究》2007年第4期245-248,共4页Chinese Ophthalmic Research

基　　金：中国国家留学基金(2004842118);湖北省科技攻关项目(2004AA301C37)资助

摘　　要：目的探讨犬尿氨酸转氨酶-Ⅱ(KAT-Ⅱ)在不同年龄的正常BL6小鼠与DBA/2J高眼压模型小鼠眼中的表达差异。方法将3、6、11月龄的BL6和DBA/2J小鼠全眼组织冰冻切片,通过免疫组织化学和DAPI双荧光染色法检测KAT-Ⅱ的表达及差异,双激发波荧光显微镜观察拍照。结果两系小鼠眼组织中,KAT-Ⅱ表达于角膜上皮、内皮,睫状体上皮和睫状肌内血管内皮,视网膜节细胞层。3、6月龄的DBA/2J小鼠眼角膜上皮和视网膜神经节细胞(RGCs)表达比BL6明显增强。BL6小鼠睫状体组织中6个月时KAT-Ⅱ表达最强,因DBA/2J睫状突发育不良而无法进行比较。结论KAT-Ⅱ在眼前节中的表达和在DBA/2J小鼠眼中的表达增强,提示KAT-Ⅱ可能参与眼压增高和视网膜变性等病理过程。Objective In tryptophan metabolism,kynurenine aminotransferas （KAT） is the key biosynthetic enzyme of kynurenic acid （KYNA）,the excitatory amino acid receptor antagonist and neurotransmission modulator. The aim of this study was to explore the ocular tissue （s） of expressing KAT subtype, KAT-Ⅱ during developement of normal BL6 mice and further compare the difference of the expression of KAT- Ⅱ between BL6 mice and DBA/2J intraocular hypertension mice. Methods Immunohistochemistry and 4,6-Diamidino-2-phenylindole dihydrochloride hydrate （DAPI）double labeling with antibody specified against KAT- Ⅱ were used to locate the distribution in ocular tissues of 9 normal BL6 mice and 9 DBA/2J mice aged 3,6,11 months and analyzed the difference of KAT-Ⅱ exprssion between two strains. Results In both mice strains,green KAT-Ⅱ positive staining was found mainly in corneal epithelial and endothelial ceils ,retinal ganglion cells layer,ciliary epithelial cells and endothelial cells of blood vessels in ciliary muscles. The comparison of these two strains revealed that there was a stronger staining of KAT-Ⅱ in corneal epithelial and retinal ganglion cells in DBA/2J during the early stages （3 and 6 months） than that in BL6. In ciliary body of BL6 mice, the KAT-Ⅱ positive staining peaked at the 6-month old mice. It was uncomparable in KAT-Ⅱ expression between two srains in ciliary body due to the undeveloped ciliary spur in DBA/2J mice. Conclusion The overexpression of KAT-Ⅱ in DBA/2J mice suggests that KAT-Ⅱ may play an importmant role in the development of ocular hypertension and retina degeneration.

关 键 词：犬尿氨酸转氨酶-Ⅱ 眼压 角膜 腱状体 视网膜 

分 类 号：R775[医药卫生—眼科] R774[医药卫生—临床医学]