喉癌组织中TSLC1基因启动子过甲基化及mRNA表达  

Promoter hypermethylation and mRNA expression of TSLC1 gene in laryngeal squamous cell carcinoma

作　　者：王军利[1] 张惠中[2] 白万胜[1] 刘丽[2] 卞卡[1] 成诗银[1] 

机构地区：[1]第四军医大学唐都医院耳鼻喉科,陕西西安710038 [2]第四军医大学唐都医院临床实验科,陕西西安710038

出　　处：《第四军医大学学报》2007年第7期651-653,共3页Journal of the Fourth Military Medical University

摘　　要：目的:探讨TSLC1基因启动子区过甲基化与喉癌的关系.方法:采用甲基化特异性PCR和RT-PCR法分析喉部正常粘膜、声带息肉、喉癌细胞Hep-2和喉癌组织中TSLC1基因启动子区过甲基化及其mRNA表达状况.结果:40例喉癌组织中,有21(52.5%)例TSLC1基因启动子区过甲基化,其在各病理分级、临床分期和分型之间差异无统计学意义(P>0.05),而在N分级(N0和N1)之间差异有统计学意义(P<0.05).喉癌细胞Hep-2也显示TSLC1基因启动子区过甲基化.RT-PCR结果显示,TSLC1 mRNA在所有甲基化的喉癌组织和喉癌细胞Hep-2中均无表达,而喉部正常组织,声带息肉组织和非甲基化的喉癌组织中均有表达.结论:喉癌组织中TSLC1基因启动子区过甲基化与其mRNA失表达有关,可能是喉癌发生、发展的原因之一,可作为喉癌诊断和预后分析的检测指标.AIM： To explore the relationship between hypermethylation of the promoter of TSLC1 gene and laryngeal squamous cell cancer. METHODS： Promoter hypermethylation and mRNA expression of TSLC1 gene were respectively detected by methylation-speeific PCR and RT-PCR in normal laryngeal mucosa （n = 4） and laryngeal squamous cell cancer （n = 40）. RESULTS： Among the 40 patients with laryngeal squamous cell cancer, hypermethylation of TSLC1 promoter was seen in 21 cases （52.5%）. There was no significant difference in hypermethylation in relation to pathological grade and clinical stage and classification （P 〉 0. 05 ）, but a highly significant difference was observed between the patients with and without lymph node metastasis （ NO and N1 ） （ P 〈 0.05 ）. TSLC1 promoter hypermethylation was observed in human laryngeal squamous cell line Hep-2. TSLC1 mRNA was not expressed in all laryngeal squamous cell cancers with hypermethylation of TSLC1 promoter and Hep-2 cells, whereas it was expressed in normal laryngeal mucosa, polyp of vocal cord and laryngeal squamous cell cancers without hypermethylation. CONCLUSION： Hypermethylation of TSLC1 promoter is associated with loss of its transcription in laryngeal squamous cell carcinoma. The high- frequency hypermethylation of TSLC1 promoter illustrates its potential clinical application as tumor marker for diagnosis and prognosis.

关 键 词：TSLC1基因 喉肿瘤 启动子 甲基化 

分 类 号：R739.65[医药卫生—肿瘤]