全氟己基正辛烷眼内耐受性的实验研究  

Experimental study on intraocular tolerance of F_6H_8

作　　者：赵芳[1] 赵秉水[2] 林锦镛[2] 亢雷[2] 

机构地区：[1]天津市天和医院眼科,300050 [2]天津市眼科医院,300020

出　　处：《眼科新进展》2007年第4期278-282,共5页Recent Advances in Ophthalmology

摘　　要：目的探讨不同剂量的全氟己基正辛烷(perfluorohexyloctane,F6H8)兔眼玻璃体腔内存留不同时间对组织解剖结构和功能的影响,并评价其作为硅油清洗剂和眼内填充物的安全性。方法将20只比利时有色兔随机分为2组。小剂量组实验眼玻璃体腔注入0.2mL F6H8;大剂量组利用全氟丙烷(perfluoropropane,C3F8)气体膨胀压缩玻璃体腔后注入1.0mL F6H8;对照眼采用相同方法注入相同剂量的生理盐水。分别于术前和术后一定时间给予裂隙灯、间接检眼镜及眼压(intraocular pressure,IOP)检查;另外每组随机取4只兔分别在术前及术后2周、4周、8周和12周进行视网膜电图的重复检查,最后气栓法处死兔取眼球行光学显微镜检查。结果小剂量组:术前及术后实验眼前后节检查、IOP、视网膜电图b波波幅及光镜检查均未发现明显异常。大剂量组:实验眼和对照眼在术后1d时IOP均明显低于正常,之后逐渐回升并保持平稳;视网膜电图b波波幅呈明显下降趋势;组织病理学检查发现视网膜前泡沫细胞,视网膜前及视网膜内大量空泡样改变,外丛状层变薄甚至视网膜全层变薄,内外核层排列紊乱,色素上皮层和锥杆细胞层的明显变性以及Mller细胞、小胶质细胞的增生。结论F6H8长期玻璃体腔填充会造成视网膜毒性,可以短期或在手术中暂时使用,术后应尽量将其清除干净,玻璃体腔内残留少量的小滴并不影响视网膜的结构和功能;目前尚不能用于眼内长期填充物。Objective To investigate the effect of different dose of perfluorohexyloctane（ F6H8 ） on structure and fimction of rabbit eyes with different time remaining in cavum vitreum,and evaluate the security of F6H8 as silicone oil solvent and intravitreal implants. Methods Twenty pigmented rabbits were derided into 2 groups randomly. In small dose group,0.2 mL F6H8 was injected into the cavum vitreum in experimental right eyes;1.0 mL F6H8 was injected into the cavum vitreum after which was compressed by expanding gas of perfluoropropane in experimental right eyes in large dose group. All left eyes received the same dose of NS with same methods as control. Slit lamp biomicroscopy,indirect ophthalmoscope and intraocular pressure measurement were performed at certain time in all eyes pre- and postoperatively,and electroretinogram were performed repeatedly preoperatively at 2 weeks, 4 weeks, 8 weeks, 12 weeks postoperatively in 4 rabbits chosen randomly in each group. At the end all these rabbits were killed by air embolism,the eyeballs were enucleated and light microseopy was performed to study the structure of the retina. Results Insmall dose group, there were no visible abnormality in examination of anterior and posterior eyeball, IOP, amplitude orb wave and light microscopy in experimental eyes pre- and postoperatively. In large dose group,IOP in control and experimental eyes was lower than normal on the first day in all eyes postoperatively, then which began to increase and remained stably;The amplitude orb wave was in the tendency of decrease markedly; Preretinal foam cells, retinal thinning, structural disturbance in inner or outer nuclear layers and plexfform layers,pen- etration of all retinal layers by the vacuolus, hypertrophy of Mfiller and glial cells were found by histopathology. Conclusion F6H8 is harmful to retina if it is used for a long time as intraocular implants, and it can be used in a short time or in surgery temporarily,but which should be removed out completely after surgery.

关 键 词：全氟己基正辛烷 毒性 视网膜 动物实验 

分 类 号：R774.1[医药卫生—眼科]