鸡传染性支气管炎病毒陕西分离株M蛋白基因的变异及其B细胞表位稳定性  

Analysis of stability of B cell epitopes and variation of matrix glycoprotein gene of avian infectious bronchitis virus Shaanxi isolates

作　　者：王晶钰[1] 罗艳[1] 张彦明[1] 邓小敏[1] 姜向阳[1] 

机构地区：[1]西北农林科技大学动物科技学院,陕西杨凌712100

出　　处：《中国兽医科学》2007年第4期282-286,共5页Chinese Veterinary Science

基　　金：陕西省科技攻关项目(2004K02-G3-04);西北农林科技大学科研专项(20030203015)

摘　　要：以陕西8株鸡传染性支气管炎病毒（AIBV）分离株M蛋白的基因序列为基础，应用DNAStar软件中的MegAlign模块对其进行了同源性分析；用Kyte-Doolittle方法、Jameson—Wolf方法、Karplus—Schulz方法、Plot—Emini方法和Garnier-Robson方法综合预测了其中4株代表毒株的M蛋白B细胞抗原表位和二级结构。结果，8株AIBV的M蛋白主要在10～40aa和90～175aa处存在无规律的点突变，与参考毒株H52、M41、SAIB20、LX和Gray的M蛋白的核苷酸序列和预测的氨基酸序列的同源性分别介于87．4％～99．5％和96．3％～99．5％；分离株间的核苷酸序列和预测的氨基酸序列同源性分别介于91．8％～99．8％和95．4％～100％。4株代表株的M蛋白B细胞优势抗原表位都在159～164aa和181～193aa肽段区（这2个区域都包含了无规则卷曲和β-转角结构）或其附近。结果表明，AIBV分离株的M蛋白基因相对保守，只存在无规律的点突变，该变异对AIBVM蛋白B细胞表位的稳定性无影响。Similarities chitis virus isolated from ware,and then the B cell of matrix（M） pr Shaanxi Province otein gene sequences among was analysed by MegAlign epitopes and secondary structure of M protein Jameson-Wolf, Karplus-Schulz, Plot-Emini a deduced amino acid sequence of M protein o aa and 90-175 aa. Furthermore -99.5% similar at amino acid shared 91.8%-99.8% and 95. tein of 4 representative isolates the 8 isolat level. With 4%-100% were aa） with irregular coil and β-turn relatively conserved,and the varia or 8 isolates of avian infectious bronmodule of DNAStar biologic softwere predicted by Kyte-Doolittle, nd Garnier-Robson methods. Ruleless point-mutations of the f the 8 isolates were localized mainly in the N-terminal 10-40 es were 87.4%-99.5% similar at nucleotide level and 96.3% the reference strains H52, M41, SAIB20, LX and Gray, they identity, respectively. Moreover,the B cell epitopes of M prolocated near th tions have in the shared regio em. These results no effect on the sta ns （N-terminal 159-164 aa and 181-193 showed that the AIBV M protein gene is bility of B cell epitopes of M protein.

关 键 词：鸡传染性支气管炎病毒 M蛋白 基因变异 B细胞表位 稳定性 

分 类 号：S852.65[农业科学—基础兽医学]