通心络超微粉对高脂饮食兔主动脉内皮保护机制的实验研究  

The protective effect of tongxinluo supermicropowder on aorta endodermis in rabbits fed with fatty

作　　者：吴以岭[1] 袁国强[1] 游佳华[1] 梁俊清[1] 贾振华[1] 魏聪[1] 

机构地区：[1]河北医科大学医药研究院,河北石家庄050035

出　　处：《中国病理生理杂志》2007年第4期629-633,共5页Chinese Journal of Pathophysiology

基　　金：国家重点基础研究发展计划(973计划)项目资助(No.2005CB523301)

摘　　要：目的:探讨通心络超微粉对高脂饮食兔主动脉内皮损伤的干预作用及其可能机制。方法:健康雄性新西兰白兔32只随机分为空白对照组、模型组、阿托伐他汀组、通心络组4组。空白对照组饲以普通饲料;模型组饲以高脂饲料;阿托伐他汀组饲以高脂饲料同时阿托伐他汀3 mg.kg-1.d-1灌胃;通心络组饲以高脂饲料同时通心络超微粉0.31 g.kg-1.d-1灌胃,连续给药,于6周末酶法检测各组血脂水平,比色法检测各组血清NO、MDA水平及SOD活性,免疫组织化学染色法检测主动脉内皮细胞NF-κB核转位情况及ICAM-1蛋白表达,RT-PCR法检测ICAM-1 mRNA表达。结果:模型组血清TC、TG、LDL-C、HDL-C及MDA水平均显著高于空白对照组(P<0.01),NO水平及SOD活性低于空白组(P<0.01);主动脉内皮细胞NF-κB核转位、ICAM-1基因及蛋白表达明显多于空白组(P<0.01)。两药物干预组TC、TG、LDL-C及MDA水平均显著低于模型组(P<0.01),NO水平和SOD活性则高于模型组(P<0.05,P<0.01),主动脉内皮细胞NF-κB核转位、ICAM-1基因及蛋白表达亦明显少于模型组(P<0.05,P<0.01),通心络组HDL-C显著高于模型组(P<0.05),且通心络组对上述指标的作用均优于阿托伐他汀组(P<0.05,P<0.01)。结论:①高脂血症可使血管内皮细胞受损,其机制可能与氧化应激、NF-κB的激活及ICAM-1基因与蛋白的表达异常有关。②通心络超微粉可在一定程度上减轻上述病理损伤,可能与其抗氧化、抑制NF-κB核转位进而降低ICAM-1基因及蛋白表达有关。AIM： To discuss the tongxinluo supermicropowder＇s interventional effect on aorta endodermis of rabbits which are fed with fatty forage plants. METHODS ： Thirty - two healthy male New Zealand rabbits were divided into 4 groups randomly： control group, model group, atorvastatin treatment group, and tongxinluo treatment group. The control group was fed with common feedstuff, and all the other groups were fed with fatty feedstuff. The atorvastatin treatment group and the tongxinluo treatment group were given suspension of atorvastatin （3 g·kg^-1·d-1） and tongxinluo supermicropowder（0. 31 g·kg^-1·d-1） by intragastric administration on the basis of fatty feedstuff. All the groups were fed with medicine for 6 weeks. At the end of 6 weeks, blood lipid levels were observed by enzymic method, the levels of blood serum NO and MDA and the activity of SOD were observed by chromatometry, the expression of nuclear factor - kappaB （ NF - κB）, intercellular adhesion molecule - 1 （ ICAM - 1 ） was detected, and VCAM - 1 mRNA expression in the aortic tissue was examined by reverse transcription polymerase chain reaction（ RT -PCR）. RESULTS： The levels of blood serum TC, TG, LDL- C, HDL- C, and MDA were increased significantly compared with the control group（ P 〈 0.01 ） ; the level of NO and the activity of SOD were decreased compared with control group（P 〈0.01 ） ; NF - κB, the expression of VCAM - 1 gene and protein were increased significantly compared with control group（ P 〈 0. 01 ）. In the two medicine interventional groups, levels of TC, TG, LDL - C and MDA were significantly decreased compared with model group, the level of NO and the activity of SOD were higher than those in model group（P 〈0. 05, P 〈0.01 ）, NF - κB, the expression of VCAM - 1 gene and protein were significantly decreased compared with model group （ P 〈 0. 05 ,P 〈 0.01 ）, HDL - C of tongxinluo treatment group was significantly higher than that in model group（ P 〈 0. 05）, and the effect

关 键 词：通心络 内皮 血管 氧化性应激 NF-κB 胞间粘附分子1 

分 类 号：R363[医药卫生—病理学]