菊米提取液舒张血管作用及其机制研究  

Vasodilatation induced by Jumi extraction and its mechanisms

作　　者：叶挺梅[1] 徐和靖[2] 汪洋[2] 朱立[3] 沈岳良[3] 陈莹莹[3] 

机构地区：[1]丽水学院化学与生命科学院生物系,浙江丽水323000 [2]温州医学院生理教研室,浙江温州325027 [3]浙江大学医学院生理学教研室,浙江杭州310006

出　　处：《中国病理生理杂志》2007年第4期644-647,共4页Chinese Journal of Pathophysiology

基　　金：浙江省科技厅基金资助项目(No.2004C33104)

摘　　要：目的:研究菊米的舒张血管作用以及其作用机制。方法:采用血管环灌流装置测定离体大鼠胸主动脉环的血管舒张效应。结果:菊米提取液(0.5-8 g/L)可引起主动脉环发生浓度依赖性舒张。且内皮完整组的舒张程度明显大于去内皮组。用L-NAME或KCl(20 mmol/L)孵育内皮完整的血管环后,可明显抑制菊米提取液引发的血管舒张。去内皮的血管环用L-NAME孵育后,菊米提取液引发的血管舒张作用明显减弱。含有完整内皮或去内皮的胸主动脉用菊米提取液(8 g/L)孵育30 min后,组织中NOS的活性均大于未用菊米提取液孵育的对照组。结论:菊米提取液可引起血管发生内皮依赖性和非内皮依赖性的舒张作用。其作用机制可能与增加NOS活性和内皮细胞超极化因子(EDHF)释放增加有关。AIM： The objectives of the present study were to examine the effect of Jumi （JM） extraction on relaxation of isolated rat aortic rings, and to elucidate its mechanisms. METHODS： The thoracic aortic rings with and without endothelium of male Sprague - Dawley rats were mounted on a bath system. Vasodilatation of aortic rings preconstricted with 10^-6 mol/L of phenylephrine （PE） was measured. RESULTS： JM extraction （0. 5 -8 g/L） caused a concentration - dependent relaxation in aortic rings. The extent of relaxation was larger in endothelium - intact aortic rings than that in endothelium - denuded aortic rings. Both L- NAME [a nitric oxide synthase （NOS） inhibitor] and high potassium （20 mmol/L KCI） partly abolished the relaxation action of JM extraction in endothelium - intact aortic rings. Pretreatment with L - NAME also inhibited the relaxation response to JM extraction in endothelium - denuded aortic rings. After incubation with JM extraction, NOS activities enhanced both in endothelium - intact and endnthelium - denuded aortic rings. CONCLUSION： JM extraction causes relaxation of aortic rings through endothelium - dependent and independent pathways. The mechanisms might be involved in NOS and endothelium - derived hyperpolarizing factor.

关 键 词：菊米提取液 血管舒张 一氧化氮合酶 

分 类 号：Q463[生物学—生理学]